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Literature summary for 3.4.21.4 extracted from

  • Barkia, A.; Bougatef, A.; Nasri, R.; Fetoui, E.; Balti, R.; Nasri, M.
    Trypsin from the viscera of Bogue (Boops boops): isolation and characterisation (2009), Fish Physiol. Biochem., 36, 893-902.
    View publication on PubMed
Search for more literature for 3.4.21.4

Application

Application Comment Organism
food industry the enzyme can be used as a possible biotechnological tool in the fish processing and food industries Boops boops

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 14% inhibition at 5 mM Boops boops
EDTA 12% inhibition at 5 mM Boops boops
Hg2+ 72% inhibition at 5 mM Boops boops
Mg2+ 24% inhibition at 5 mM Boops boops
additional information no or poor inhibition by pepstatin A, 2-mercaptoethanol, Ca2+, K+, and Na+ Boops boops
NaCl 60& inhibition at 35% NaCl Boops boops
PMSF 62% inhibition at 1 mM, complete inhibition at 5 mM Boops boops
Soybean trypsin inhibitor complete inhibition at 1 mg/ml Boops boops
Zn2+ 18% inhibition at 5 mM Boops boops

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 9.0, 25°C Boops boops

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 gel filtration and native PAGE Boops boops

Organism

Organism UniProt Comment Textmining
Boops boops
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 8.5fold from viscera by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography Boops boops

Source Tissue

Source Tissue Comment Organism Textmining
viscus
-
 Boops boops
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.2
-
 purified enzyme, pH 9.0, 25°C Boops boops

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
 Boops boops ?
-
 ?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
-
 Boops boops N-benzoyl-DL-arginine + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 23000, SDS-PAGE Boops boops

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 substrate N-benzoyl-DL-arginine 4-nitroanilide Boops boops

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 50% of maximal activity at 40°C and 60°C, inactive at 70°C Boops boops

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 60 min, purified enzyme, pH 9.0, completely stable Boops boops
40
-
 60 min, purified enzyme, pH 9.0, 80% activity remaining Boops boops
50
-
 60 min, purified enzyme, pH 9.0, 55% activity remaining Boops boops
55
-
 60 min, purified enzyme, pH 9.0, 25% activity remaining Boops boops
60
-
 15 min, purified enzyme, pH 9.0, inactivation Boops boops

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.56
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 9.0, 25°C Boops boops

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 substrate N-benzoyl-DL-arginine 4-nitroanilide Boops boops

pH Range

pH Minimum pH Maximum Comment Organism
6 11 activity range, 38.3% of maximal activity at pH 11.0 Boops boops

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 12 completely stable at pH 7.0-9.0, 72% and 57% activity remaining at pH 11.0 and pH 12.0 Boops boops

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 9.0, 25°C Boops boops