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Literature summary for 3.4.21.4 extracted from

  • Silva, L.B.; Reis, A.P.; Pereira, E.J.; Oliveira, M.G.; Guedes, R.N.
    Partial purification and characterization of trypsin-like proteinases from insecticide-resistant and -susceptible strains of the maize weevil, Sitophilus zeamais (2010), Comp. Biochem. Physiol. B, 155, 12-19.
    View publication on PubMed
Search for more literature for 3.4.21.4

Inhibitors

Inhibitors Comment Organism Structure
Aprotinin
-
 Sitophilus zeamais
benzamidine
-
 Sitophilus zeamais
Bowman-Birk inhibitor
-
 Sitophilus zeamais
E64 weak inhibition Sitophilus zeamais
leupeptin
-
 Sitophilus zeamais
N-alpha-tosyl-L-lysine chloromethyl ketone
-
 Sitophilus zeamais
pepstatin
-
 Sitophilus zeamais
phenylmethyl sulfonyl fluoride
-
 Sitophilus zeamais
Soybean trypsin inhibitor
-
 Sitophilus zeamais

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Sitophilus zeamais

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, best at 20 mM Sitophilus zeamais

Organism

Organism UniProt Comment Textmining
Sitophilus zeamais
-
 insecticide-resistant and -susceptible strains, serine proteinases with trypsin-like activity
-

Purification (Commentary)

Purification (Comment) Organism
native enzymes partially by aprotinin affinity chromatography, 51.2fold Sitophilus zeamais

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
529 875 partially purified native trypsin-like serine proteinases, pH 8.2, 25°C Sitophilus zeamais

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Sitophilus zeamais ?
-
 ?
N-alpha-p-tosyl-L-Arg methyl ester + H2O
-
 Sitophilus zeamais N-alpha-p-tosyl-L-Arg + methanol
-
 ?
N-benzoyl-L-arginine 4-nitroanilide + H2O the trypsin-like proteinases from the resistant strains exhibited higher affinity for the substrate than the enzymes from the susceptible strains Sitophilus zeamais N-benzoyl-L-arginine + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
trypsin-like serine proteinase
-
 Sitophilus zeamais

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Sitophilus zeamais

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 65 assay range Sitophilus zeamais

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Sitophilus zeamais

pH Range

pH Minimum pH Maximum Comment Organism
4 10 assay range Sitophilus zeamais

General Information

General Information Comment Organism
additional information enhanced serine proteinase activity may be playing a role in mitigating physiological costs associated with the maintenance of insecticide resistance mechanisms in some maize weevil strains Sitophilus zeamais