Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
the denatured trypsin is diluted with 1 M diethanolamine or other solutions to a final concentration of 100 nM at pH 8.2, in the case of fully reduced trypsin, the refolding solution also contains 3 mM glutathione and 1 mM oxidized glutathione, the addition of 1.5 M urea resulted in even less recovered activity despite counteracting the formation of precipitates, refolding from fully reduced trypsin is achieved with a substantial approximate 86% recovery of protein yield | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-alpha-p-tosyl-L-arginine methyl ester + H2O | - |
Bos taurus | N-alpha-p-tosyl-L-arginine + methanol | - |
? |