Activating Compound | Comment | Organism | Structure |
---|---|---|---|
caerulein | trypsinogen activation to trypsin occurs with 10 nM caerulein, trypsinogen is activated to reach a trypsin level of 6 nM following 10 nM caerulein induction for 1 h | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
leupeptin | - |
Homo sapiens | |
Soybean trypsin inhibitor | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00399 | - |
enhanced green fluorescent protein-T1 | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens | |
0.00421 | - |
enhanced green fluorescent protein-T1nb | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens | |
0.00927 | - |
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens | |
0.07744 | - |
benzoyl-DL-Arg-7-amido-4-methylcoumarin | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens | |
3 | - |
benzoyl-DL-Arg-p-nitroanilide | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
enhanced green fluorescent protein-T1 + H2O | Homo sapiens | enhanced green fluorescent protein-T1 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 20 and 8 kDa | ? | - |
? | |
enhanced green fluorescent protein-T2 + H2O | Homo sapiens | enhanced green fluorescent protein-T2 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 18 and 10 kDa | ? | - |
? | |
additional information | Homo sapiens | does not cleave enhanced green fluorescent protein-T1m and wild type enhanced green fluorescent protein | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MiaPaCa-2 cell | - |
Homo sapiens | - |
pancreatic cancer cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-DL-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzoyl-DL-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzoyl-DL-Arg-p-nitroanilide + H2O | - |
Homo sapiens | benzoyl-DL-Arg + p-nitroaniline | - |
? | |
enhanced green fluorescent protein-T1 + H2O | enhanced green fluorescent protein-T1 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 20 and 8 kDa | Homo sapiens | ? | - |
? | |
enhanced green fluorescent protein-T1nb + H2O | - |
Homo sapiens | ? | - |
? | |
enhanced green fluorescent protein-T2 + H2O | enhanced green fluorescent protein-T2 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 18 and 10 kDa | Homo sapiens | ? | - |
? | |
additional information | does not cleave enhanced green fluorescent protein-T1m and wild type enhanced green fluorescent protein | Homo sapiens | ? | - |
? | |
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg + 7-amino-4-methylcoumarin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
benzoyl-DL-Arg-7-amido-4-methylcoumarin | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens | |
0.26 | - |
enhanced green fluorescent protein-T1 | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens | |
0.85 | - |
enhanced green fluorescent protein-T1nb | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens | |
1.33 | - |
benzoyl-DL-Arg-p-nitroanilide | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens | |
1.41 | - |
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | enhanced green fluorescent protein-T1 can be cleaved by trypsin under buffer conditions from pH 5 to pH 9 | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000031 | - |
leupeptin | in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C | Homo sapiens |