Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3)pLysS | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R193A | slightly reduced activity | Homo sapiens |
R193G | completely resistant to chymotryptic degradation | Homo sapiens |
R193G/G142A | reduced activity | Homo sapiens |
R193G/G184A | reduced activity | Homo sapiens |
R193G/G19A | reduced activity, completely resistant to chymotryptic degradation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | trypsin 4 is completely resistant to chymotryptic degradation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0141 | - |
Z-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193G/G142A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
0.0203 | - |
Z-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193G, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
0.0266 | - |
Z-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
0.0298 | - |
Z-Gly-Pro-Arg-p-nitroanilide | wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
0.0788 | - |
Z-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193G/G19A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
0.346 | - |
Z-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193G/G184A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose Fast Flow column chromatography and Phenyl Sepharose Fast Flow column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-4-guanidinobenzoate + H2O | - |
Homo sapiens | 4-methylumbelliferol + 4-guanidinobenzoate | - |
? | |
Z-Gly-Pro-Arg-p-nitroanilide + H2O | - |
Homo sapiens | Z-Gly-Pro-Arg + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trypsin 4 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.86 | - |
Z-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193G/G142A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
20 | 50 | Z-Gly-Pro-Arg-p-nitroanilide | wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
29.2 | - |
Z-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193G/G184A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
51.5 | - |
Z-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193G/G19A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
54.1 | - |
Z-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193G, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
83.6 | - |
Z-Gly-Pro-Arg-p-nitroanilide | mutant enzyme R193A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens | |
88.2 | - |
Z-Gly-Pro-Arg-p-nitroanilide | wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C | Homo sapiens |