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Literature summary for 3.4.21.4 extracted from
- Penaeus vannamei isotrypsins: purification and characterization (2004), Comp. Biochem. Physiol. B, 138B, 155-162.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phenylmethylsulfonyl fluoride | - |
Penaeus vannamei |  |
| Soybean trypsin inhibitor | - |
Penaeus vannamei | |
| tosyl-L-Phe chloromethyl ketone | - |
Penaeus vannamei | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | highest activity in presence of 30-50 mM, isoforms A, B, C | Penaeus vannamei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30200 | - |
x * 32900, isoforms A, B, x * 30200, isoform C, SDS-PAGE | Penaeus vannamei |
| 32900 | - |
x * 32900, isoforms A, B, x * 30200, isoform C, SDS-PAGE | Penaeus vannamei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penaeus vannamei | - |
isoforms A,B,C | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | isoforms A, B, and C contain both glucosamine and galactosamine residues | Penaeus vannamei |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoforms A, B, C | Penaeus vannamei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive gland | - |
Penaeus vannamei | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32900, isoforms A, B, x * 30200, isoform C, SDS-PAGE | Penaeus vannamei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
isoforms A,B and C | Penaeus vannamei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 20 | less than 20% of maximum activity, isoforms A,B, and C | Penaeus vannamei |
| 60 | - |
above, sharp decrease in activity, isoforms A,B, and C | Penaeus vannamei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | isoforms A, B, C, more than 80% of maximum activity within this range | Penaeus vannamei |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Penaeus vannamei | isoelectric focusing, isoform B | - |
3 |
| Penaeus vannamei | isoelectric focusing, isoform A | - |
3.5 |
| Penaeus vannamei | isoelectric focusing, isoform C | - |
4.5 |
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