Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.4 extracted from

  • Reyda, S.; Sohn, C.; Klebe, G.; Rall, K.; Ullmann, D.; Jakubke, H.D.; Stubbs, M.T.
    Reconstructing the binding site of factor Xa in trypsin reveals ligand-induced structural plasticity (2003), J. Mol. Biol., 325, 963-977.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme,variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa, and variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172,Ser173, Phe174, Ile175) and Ala190 of factor Xa, expression in yeast Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of variant X99rT containing the 99-loop (Glu97, Tyr99) of factor Xa, and variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa obtained in presence of benzamidine Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one
-
Rattus norvegicus
benzamidine
-
Rattus norvegicus
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester
-
Rattus norvegicus
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
Pefachrome tPA native enzyme Rattus norvegicus
0.0229
-
Pefachrome tPA variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa Rattus norvegicus
0.0472
-
Pefachrome tPA variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172,Ser173, Phe174, Ile175) and Ala190 of factor Xa Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Pefachrome tPA + H2O
-
Rattus norvegicus ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000805
-
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester native enzyme Rattus norvegicus
0.0000247
-
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa Rattus norvegicus
0.000054
-
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa Rattus norvegicus
0.000122
-
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one native enzyme Rattus norvegicus
0.00152
-
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester variant X99rT containing the 99-loop (Glu97, Tyr99) of factor Xa Rattus norvegicus
0.00605
-
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa Rattus norvegicus
0.00651
-
benzamidine native enzyme Rattus norvegicus
0.00871
-
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172, Ser173, Phe174, Ile175) and Ala190 of factor Xa Rattus norvegicus
0.0256
-
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone native enzyme Rattus norvegicus
0.0322
-
benzamidine variant X99rT containing the 99-loop (Glu97, Tyr99) of factor Xa Rattus norvegicus
0.0445
-
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa Rattus norvegicus
0.143
-
benzamidine variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172,Ser173, Phe174, Ile175) and Ala190 of factor Xa Rattus norvegicus