Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | precipitation of alpha-chymotrypsin in the simultaneous presence of ammonium sulfate and tert-butanol, i.e. three phase partitioning, results in preparations which show self aggregation of the enzyme molecules. The aggregates have irregular shapes and have about 3fold higher catalytic activity than the native enzyme. Results indicate that hydrophobic interactions were the driving force behind this aggregation | Bos taurus |
Organic Solvent | Comment | Organism |
---|---|---|
tert-Butanol | precipitation of alpha-chymotrypsin in the simultaneous presence of ammonium sulfate and tert-butanol, i.e. three phase partitioning, results in preparations which show self aggregation of the enzyme molecules. The aggregates have irregular shapes and have about 3fold higher catalytic activity than the native enzyme. The aggregates do not differ in lambdamax of fluorescence emission, which is around 340 nm. All the aggregates show higher fluorescence emission intensity. Far-UV and near-UV circular dichroism also show no significant structural changes as compared to the native molecule. HPLC gel filtration gives 14 nm as the diameter for all preparations. Results indicate that hydrophobic interactions were the driving force behind this aggregation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00766 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
pancreas | - |
Bos taurus | - |