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Literature summary for 3.4.21.1 extracted from

  • Tamaki, F.K.; Padilha, M.H.; Pimentel, A.C.; Ribeiro, A.F.; Terra, W.R.
    Properties and secretory mechanism of Musca domestica digestive chymotrypsin and its relation with Drosophila melanogaster homologs (2012), Insect Biochem. Mol. Biol., 42, 482-490.
    View publication on PubMed
Search for more literature for 3.4.21.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Musca domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin pH 8.5, temperature not specified in the publication Musca domestica
0.012
-
 N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin pH 8.5, temperature not specified in the publication Musca domestica
0.0346
-
 N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide pH 8.5, temperature not specified in the publication Musca domestica
0.112
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 8.5, temperature not specified in the publication Musca domestica

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Musca domestica
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Musca domestica

Organism

Organism UniProt Comment Textmining
Musca domestica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Musca domestica

Source Tissue

Source Tissue Comment Organism Textmining
midgut posterior midgut Musca domestica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information primary specificity (kcat/Km) for Phe rather than Tyr and Leu residues. No substrates: short substrates such as N-succinyl-Phe-7-amido-4-methylcoumarin Musca domestica ?
-
 ?
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
-
 Musca domestica N-succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Musca domestica N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
-
 Musca domestica N-succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Musca domestica N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Musca domestica

Synonyms

Synonyms Comment Organism
CHY1
-
 Musca domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.55
-
 N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide pH 8.5, temperature not specified in the publication Musca domestica
3.8
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 8.5, temperature not specified in the publication Musca domestica
23.9
-
 N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin pH 8.5, temperature not specified in the publication Musca domestica
42
-
 N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin pH 8.5, temperature not specified in the publication Musca domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Musca domestica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16
-
 N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide pH 8.5, temperature not specified in the publication Musca domestica
34
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 8.5, temperature not specified in the publication Musca domestica
2000
-
 N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin pH 8.5, temperature not specified in the publication Musca domestica
3500
-
 N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin pH 8.5, temperature not specified in the publication Musca domestica