Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BPTI | basic pancreatic trypsin inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected | Struthio camelus | |
CMTI I | Cucurbita maxima trypsin inhibitor, unusual interaction with the highly selective protein trypsin inhibitor from Cucurbita maxima, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect | Struthio camelus | |
CMTI IV | Cucurbita maxima trypsin inhibitor, unusual interaction with the highly selective protein trypsin inhibitor from Cucurbita maxima, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect | Struthio camelus | |
CPGT I | Cucurbita pepo var. giramontia inhibitor, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed | Struthio camelus | |
CPTI IV | Cucurbita pepo inhibitor, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed | Struthio camelus | |
CyPTI VII | trypsin inhibitors from Cyclanthera pedata, Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition. With 0.5 M NaCl inhibition observed | Struthio camelus | |
LUTI | Linum usitatissimum inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8, with 0.5 M NaCl increased inhibitory effect | Struthio camelus | |
additional information | affinity of Chtr1 to inhibitors containing Arg at P1 position. N-alpha-tosyl-L-lysine chloromethyl ketone (TLCK), Chtr1 in 0.1 M Tris-HCl, pH 7.8: no inhibition | Struthio camelus | |
STI | soybean trypsin inhibitor, inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected | Struthio camelus | |
tosyl-L-phenylalanine chloromethyl ketone | TPCK. Inhibition of Chtr1 in 0.1 M Tris-HCl, pH 7.8. With 0.5 M NaCl no inhibition detected | Struthio camelus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | chymotrypsin 4 | Struthio camelus | |
0.939 | - |
N-alpha-benzoyl-DL-arginine-p-nitroanilide | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
SDS-PAGE | Struthio camelus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Struthio camelus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Struthio camelus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
pancreas | - |
Struthio camelus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
128 | - |
activated extract | Struthio camelus |
500 | - |
affinity chromatography on CMTI in the presence of 5 M NaCl, peak B | Struthio camelus |
1573 | - |
affinity chromatography on CMTI (isolation of Chtr1) | Struthio camelus |
1800 | - |
affinity chromatography on CMTI in the presence of 5 M NaCl, peak A | Struthio camelus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | specificity of Chtr1 reactive site is determined by hydrolysis of the oxidized bovine insulin B-chain | Bos taurus | ? | - |
? | |
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O | BApNA | Bos taurus | ? + p-nitroaniline | - |
? | |
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O | BApNA | Struthio camelus | ? + p-nitroaniline | - |
? | |
N-succinyl-Ala-Ala-Ala p-nitroanilide + H2O | - |
Bos taurus | N-succinyl-Ala-Ala-Ala + p-nitroaniline | - |
? | |
N-succinyl-Ala-Ala-Ala-p-nitroanilide + H2O | - |
Struthio camelus | N-succinyl-Ala-Ala-Ala + p-nitroaniline | - |
? | |
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O | SucAAPFpNA | Bos taurus | N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline | - |
? | |
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O | SucAAPFpNA | Struthio camelus | N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Chtr1 | - |
Struthio camelus |
Chtr2 | - |
Struthio camelus |
Chtr3 | - |
Struthio camelus |
Chtr4 | - |
Struthio camelus |
chymotrypsin | - |
Bos taurus |
chymotrypsin | - |
Struthio camelus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.611 | - |
N-alpha-benzoyl-DL-arginine-p-nitroanilide | - |
Bos taurus | |
10.58 | - |
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | chymotrypsin 4 | Struthio camelus |