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Literature summary for 3.4.21.1 extracted from
- Enzymatic activity and thermal stability of PEG-alpha-chymotrypsin conjugates (2009), Biotechnol. Lett., 31, 883-887.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | alpha-chymotrypsin is chemically modified with methoxypoly(ethylene glycol) (PEG) of different molecular weights (700 (PEG700-alpha-chymotrypsin), 2000 (PEG2000-alpha-chymotrypsin), and 5000 Da (PEG5000-alpha-chymotrypsin)) and the amount of polymer attached to the enzyme is varied systematically from 1 to 9 PEG molecules per enzyme molecule | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | alpha-chymotrypsin, unmodified | Bos taurus |  |
| 0.07 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 2.2 | Bos taurus | |
| 0.07 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG700-alpha-chymotrypsin, degree of PEGylation: 3.8 | Bos taurus | |
| 0.08 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 1.3 | Bos taurus | |
| 0.08 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 2.9 | Bos taurus | |
| 0.08 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 4.2 | Bos taurus | |
| 0.08 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 6.0 | Bos taurus | |
| 0.09 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 4.2 | Bos taurus | |
| 0.1 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 1.3 | Bos taurus | |
| 0.11 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 7.6 | Bos taurus | |
| 0.15 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 8.4 | Bos taurus | |
| 0.15 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG700-alpha-chymotrypsin, degree of PEGylation: 7.9 | Bos taurus | |
| 0.19 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 9.1 | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Bos taurus | - |
| pancreas | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Bos taurus | N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-chymotrypsin | - |
Bos taurus |
| alpha-CT | - |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.2 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG700-alpha-chymotrypsin, degree of PEGylation: 7.9 | Bos taurus | |
| 7.6 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 4.2 | Bos taurus | |
| 7.9 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 9.1 | Bos taurus | |
| 8.3 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 6.0 | Bos taurus | |
| 8.6 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 7.6 | Bos taurus | |
| 8.7 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 4.2 | Bos taurus | |
| 9.4 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 8.4 | Bos taurus | |
| 10.2 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 2.9 | Bos taurus | |
| 10.7 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 2.2 | Bos taurus | |
| 11.6 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG700-alpha-chymotrypsin, degree of PEGylation: 3.8 | Bos taurus | |
| 12.1 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | alpha-chymotrypsin, unmodified | Bos taurus | |
| 13.1 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 1.3 | Bos taurus | |
| 13.5 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 1.3 | Bos taurus | |
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