Protein Variants | Comment | Organism |
---|---|---|
additional information | alpha-chymotrypsin is chemically modified with methoxypoly(ethylene glycol) (PEG) of different molecular weights (700 (PEG700-alpha-chymotrypsin), 2000 (PEG2000-alpha-chymotrypsin), and 5000 Da (PEG5000-alpha-chymotrypsin)) and the amount of polymer attached to the enzyme is varied systematically from 1 to 9 PEG molecules per enzyme molecule | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | alpha-chymotrypsin, unmodified | Bos taurus | |
0.07 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 2.2 | Bos taurus | |
0.07 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG700-alpha-chymotrypsin, degree of PEGylation: 3.8 | Bos taurus | |
0.08 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 1.3 | Bos taurus | |
0.08 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 2.9 | Bos taurus | |
0.08 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 4.2 | Bos taurus | |
0.08 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 6.0 | Bos taurus | |
0.09 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 4.2 | Bos taurus | |
0.1 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 1.3 | Bos taurus | |
0.11 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 7.6 | Bos taurus | |
0.15 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 8.4 | Bos taurus | |
0.15 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG700-alpha-chymotrypsin, degree of PEGylation: 7.9 | Bos taurus | |
0.19 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 9.1 | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Bos taurus | N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-chymotrypsin | - |
Bos taurus |
alpha-CT | - |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.2 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG700-alpha-chymotrypsin, degree of PEGylation: 7.9 | Bos taurus | |
7.6 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 4.2 | Bos taurus | |
7.9 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 9.1 | Bos taurus | |
8.3 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 6.0 | Bos taurus | |
8.6 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 7.6 | Bos taurus | |
8.7 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 4.2 | Bos taurus | |
9.4 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 8.4 | Bos taurus | |
10.2 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 2.9 | Bos taurus | |
10.7 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 2.2 | Bos taurus | |
11.6 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG700-alpha-chymotrypsin, degree of PEGylation: 3.8 | Bos taurus | |
12.1 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | alpha-chymotrypsin, unmodified | Bos taurus | |
13.1 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG5000-alpha-chymotrypsin, degree of PEGylation: 1.3 | Bos taurus | |
13.5 | - |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide | PEG2000-alpha-chymotrypsin, degree of PEGylation: 1.3 | Bos taurus |