Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.1 extracted from

  • Lopes, A.R.; Sato, P.M.; Terra, W.R.
    Insect chymotrypsins: chloromethyl ketone inactivation and substrate specificity relative to possible coevolutional adaptation of insects and plants (2009), Arch. Insect Biochem. Physiol., 70, 188-203.
    View publication on PubMed
Search for more literature for 3.4.21.1

Inhibitors

Inhibitors Comment Organism Structure
N-alpha-tosyl-L-Phe chloromethyl ketone TPCK Bos taurus
N-alpha-tosyl-L-Phe chloromethyl ketone TPCK Diatraea saccharalis
N-alpha-tosyl-L-Phe chloromethyl ketone TPCK Periplaneta americana
N-alpha-tosyl-L-Phe chloromethyl ketone TPCK Spodoptera frugiperda
N-alpha-tosyl-L-Phe chloromethyl ketone TPCK Tenebrio molitor
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone Z-GGF-CK Bos taurus
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone Z-GGF-CK Diatraea saccharalis
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone Z-GGF-CK Periplaneta americana
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone Z-GGF-CK Spodoptera frugiperda
N-carbobenzoxy-Gly-Gly-Phe-chloromethyl ketone Z-GGF-CK Tenebrio molitor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
-
 Diatraea saccharalis
0.006
-
 N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
-
 Periplaneta americana
0.01
-
 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
 Periplaneta americana
0.01
-
 N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
-
 Tenebrio molitor
0.013
-
 N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
-
 Bos taurus
0.022
-
 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
 Bos taurus
0.022
-
 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
 Diatraea saccharalis
0.023
-
 N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
-
 Spodoptera frugiperda
0.025
-
 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
 Spodoptera frugiperda
0.032
-
 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin
-
 Tenebrio molitor
0.2
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
 Spodoptera frugiperda
0.3
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
 Tenebrio molitor
0.5
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
 Bos taurus
0.5
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
 Diatraea saccharalis
1.1
-
 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
 Periplaneta americana
2.3
-
 N-succinyl-Phe-7-amido-4-methylcoumarin
-
 Tenebrio molitor
2.4
-
 N-succinyl-Phe-7-amido-4-methylcoumarin
-
 Spodoptera frugiperda
3
-
 N-succinyl-Phe-7-amido-4-methylcoumarin
-
 Bos taurus
3
-
 N-succinyl-Phe-7-amido-4-methylcoumarin
-
 Diatraea saccharalis
5
-
 N-succinyl-Phe-7-amido-4-methylcoumarin
-
 Periplaneta americana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 SDS-PAGE of partial purified chymotrypsin results in the resolution of bands of 31 and 29 kDa, in addition to others with masses lower than 14 kDa Periplaneta americana
27000
-
 SDS-PAGE Diatraea saccharalis

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Diatraea saccharalis
-
-
-
Periplaneta americana Q1M0X9
-
-
Spodoptera frugiperda Q86M89
-
-
Tenebrio molitor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Diatraea saccharalis
-
 Periplaneta americana
-
 Spodoptera frugiperda
4 insect chymotrypsins from 3 different insect orders (Dictyoptera, Coleoptera, and two Lepidoptera) are isolated using affinity chromatography Tenebrio molitor

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Bos taurus
-
midgut
-
 Tenebrio molitor
-
midgut
-
 Diatraea saccharalis
-
midgut
-
 Periplaneta americana
-
midgut
-
 Spodoptera frugiperda
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-L-Tyr p-nitroanilide + H2O
-
 Diatraea saccharalis N-benzoyl-L-tyrosine + 4-nitroaniline
-
 ?
N-benzoyl-L-Tyr-p-nitroanilide + H2O
-
 Bos taurus N-benzoyl-L-tyrosine + 4-nitroaniline
-
 ?
N-benzoyl-L-Tyr-p-nitroanilide + H2O
-
 Tenebrio molitor N-benzoyl-L-tyrosine + 4-nitroaniline
-
 ?
N-benzoyl-L-Tyr-p-nitroanilide + H2O
-
 Periplaneta americana N-benzoyl-L-tyrosine + 4-nitroaniline
-
 ?
N-benzoyl-L-Tyr-p-nitroanilide + H2O
-
 Spodoptera frugiperda N-benzoyl-tyrosine + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Bos taurus N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Tenebrio molitor N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Diatraea saccharalis N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Periplaneta americana N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Spodoptera frugiperda N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Bos taurus N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Tenebrio molitor N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Diatraea saccharalis N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Periplaneta americana N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Spodoptera frugiperda N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Bos taurus N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Tenebrio molitor N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Diatraea saccharalis N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Periplaneta americana N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Spodoptera frugiperda N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
-
 Bos taurus N-succinyl-Phe + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
-
 Tenebrio molitor N-succinyl-Phe + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
-
 Diatraea saccharalis N-succinyl-Phe + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
-
 Periplaneta americana N-succinyl-Phe + 7-amino-4-methylcoumarin
-
 ?
N-succinyl-Phe-7-amido-4-methylcoumarin + H2O
-
 Spodoptera frugiperda N-succinyl-Phe + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
chymotrypsin
-
 Bos taurus
chymotrypsin
-
 Tenebrio molitor
chymotrypsin
-
 Diatraea saccharalis
chymotrypsin
-
 Periplaneta americana
chymotrypsin
-
 Spodoptera frugiperda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Periplaneta americana
8
-
-
 Tenebrio molitor
9.8
-
-
 Diatraea saccharalis