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Literature summary for 3.4.21.1 extracted from

  • Al-Ajlan, A.; Bailey, G.S.
    Purification and characterization of cationic chymotrypsin from the pancreas of the Arabian camel (Camelus dromedarius) (2000), Mol. Cell. Biochem., 203, 73-78.
    View publication on PubMed
Search for more literature for 3.4.21.1

Inhibitors

Inhibitors Comment Organism Structure
Aprotinin
-
 Bos taurus
Aprotinin
-
 Camelus dromedarius
Lima bean trypsin inhibitor
-
 Bos taurus
Lima bean trypsin inhibitor
-
 Camelus dromedarius
Soybean trypsin inhibitor
-
 Bos taurus
Soybean trypsin inhibitor
-
 Camelus dromedarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 N-benzoyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus
0.13
-
 N-benzoyl-L-tyrosine ethyl ester 25°C, pH 7.5 Camelus dromedarius
0.14
-
 N-acetyl-L-tryptophan ethyl ester 25°C, pH 7.5 Camelus dromedarius
0.19
-
 N-acetyl-L-tryptophan ethyl ester 25°C, pH 7.5 Bos taurus
0.57
-
 N-acetyl-L-tyrosine ethyl ester 25°C, pH 7.5 Camelus dromedarius
1.12
-
 N-acetyl-L-phenylalanine ethyl ester 25°C, pH 7.5 Bos taurus
1.17
-
 N-acetyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus
2.58
-
 N-acetyl-L-phenylalanine ethyl ester 25°C, pH 7.5 Camelus dromedarius
7.02
-
 N-acetyl-L-leucine methyl ester 25°C, pH 7.5 Bos taurus
8.72
-
 N-acetyl-L-leucine methyl ester 25°C, pH 7.5 Camelus dromedarius

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Camelus dromedarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange column, cation exchange column, affinity F2 Camelus dromedarius

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-
pancreas
-
 Camelus dromedarius
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
78.4
-
 hydrolysisi of N-benzoyl-L-tyrosine ethyl ester Camelus dromedarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-leucine methyl ester + H2O 5% of kcat with N-acetyl-L-tyrosine ethyl ester Bos taurus N-acetyl-L-leucine + methanol
-
 ?
N-acetyl-L-leucine methyl ester + H2O 5% of kcat with N-acetyl-L-tyrosine ethyl ester Camelus dromedarius N-acetyl-L-leucine + methanol
-
 ?
N-acetyl-L-phenylalanine ethyl ester + H2O
-
 Bos taurus N-acetyl-L-phenylalanine + ethanol
-
 ?
N-acetyl-L-phenylalanine ethyl ester + H2O
-
 Camelus dromedarius N-acetyl-L-phenylalanine + ethanol
-
 ?
N-acetyl-L-tryptophan ethyl ester + H2O
-
 Bos taurus N-acetyl-L-tryptophan + ethanol
-
 ?
N-acetyl-L-tryptophan ethyl ester + H2O
-
 Camelus dromedarius N-acetyl-L-tryptophan + ethanol
-
 ?
N-acetyl-L-tyrosine ethyl ester + H2O
-
 Bos taurus N-acetyl-L-tyrosine + ethanol
-
 ?
N-acetyl-L-tyrosine ethyl ester + H2O
-
 Camelus dromedarius N-acetyl-L-tyrosine + ethanol
-
 ?
N-benzoyl-L-tyrosine ethyl ester + H2O
-
 Bos taurus N-benzoyl-L-tyrosine + ethanol
-
 ?
N-benzoyl-L-tyrosine ethyl ester + H2O
-
 Camelus dromedarius N-benzoyl-L-tyrosine + ethanol
-
 ?

Synonyms

Synonyms Comment Organism
alpha chymotrypsin
-
 Bos taurus
cationic chymotrypsin
-
 Camelus dromedarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 N-benzoyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus
4.6
-
 N-acetyl-L-leucine methyl ester 25°C, pH 7.5 Camelus dromedarius
6.5
-
 N-acetyl-L-leucine methyl ester 25°C, pH 7.5 Bos taurus
25.7
-
 N-acetyl-L-tryptophan ethyl ester 25°C, pH 7.5 Camelus dromedarius
40.3
-
 N-acetyl-L-phenylalanine ethyl ester 25°C, pH 7.5 Camelus dromedarius
45.9
-
 N-benzoyl-L-tyrosine ethyl ester 25°C, pH 7.5 Camelus dromedarius
48.8
-
 N-acetyl-L-tryptophan ethyl ester 25°C, pH 7.5 Bos taurus
54.8
-
 N-acetyl-L-phenylalanine ethyl ester 25°C, pH 7.5 Bos taurus
70.4
-
 N-benzoyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus
82.8
-
 N-acetyl-L-tyrosine ethyl ester 25°C, pH 7.5 Camelus dromedarius
119.5
-
 N-acetyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus
120
-
 N-acetyl-L-tyrosine ethyl ester 25°C, pH 7.5 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 hydrolysis of N-benzoyl-L-tyrosine ethyl ester Camelus dromedarius

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.8 approx. 1% of maximal activity at pH 4.5, approx. 5% of maximal activity at pH 9.8 Camelus dromedarius

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000075
-
 Aprotinin 25°C, pH 7.5 Camelus dromedarius
0.000018
-
 Aprotinin 25°C, pH 7.5 Bos taurus
0.00013
-
 Lima bean trypsin inhibitor 25°C, pH 7.5 Camelus dromedarius
0.00017
-
 Lima bean trypsin inhibitor 25°C, pH 7.5 Bos taurus
0.00067
-
 Soybean trypsin inhibitor 25°C, pH 7.5 Camelus dromedarius
0.0019
-
 Soybean trypsin inhibitor 25°C, pH 7.5 Bos taurus