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Literature summary for 3.4.21.1 extracted from

  • Sato, M.; Sasaki, T.; Kobayashi, M.; Kise, H.
    Time-dependent structure and activity changes of alpha-chymotrypsin in water/alcohol mixed solvents (2000), Biosci. Biotechnol. Biochem., 64, 2552-2558.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.23
-
N-benzoyl-L-tyrosine methyl ester transesterfication in 10% ethanol Bos taurus
3.33
-
N-benzoyl-L-tyrosine ethyl ester transesterfication in 30% methanol Bos taurus
6.02
-
N-benzoyl-L-tyrosine ethyl ester transesterfication in 10% methanol Bos taurus
20.6
-
N-benzoyl-L-tyrosine methyl ester transesterfication in 90% ethanol Bos taurus

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol at 50% ethanol alpha-chymotrypsin has an ordinary beta-sheet structure, at 70% or 90% ethanol the structure changes to a native-like, i.e. alpha and beta type, structure Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-L-tyrosine ethyl ester + H2O
-
Bos taurus N-benzoyl-L-tyrosine + ethanol
-
?
N-benzoyl-L-tyrosine ethyl ester + methanol transesteriferication Bos taurus N-benzoyl-L-tyrosine methyl ester + ethanol
-
?
N-benzoyl-L-tyrosine methyl ester + ethanol transesteriferication Bos taurus N-benzoyl-L-tyrosine ethyl ester + methanol
-
?
N-benzoyl-L-tyrosine methyl ester + H2O
-
Bos taurus N-benzoyl-L-tyrosine + methanol
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03 0.55 N-benzoyl-L-tyrosine ethyl ester transesterfication in 10% methanol Bos taurus
0.031 0.51 N-benzoyl-L-tyrosine ethyl ester transesterfication in 30% methanol Bos taurus
2.34
-
N-benzoyl-L-tyrosine methyl ester transesterfication in 10% ethanol Bos taurus
2.94
-
N-benzoyl-L-tyrosine methyl ester transesterfication in 10% ethanol Bos taurus
6.77
-
N-benzoyl-L-tyrosine ethyl ester transesterfication in 10% methanol Bos taurus
8.13
-
N-benzoyl-L-tyrosine ethyl ester transesterfication in 30% methanol Bos taurus
10.8
-
N-benzoyl-L-tyrosine methyl ester transesterfication in 90% ethanol Bos taurus