Application | Comment | Organism |
---|---|---|
additional information | PTD012 might not belong to the carboxypeptidase family of enzymes, might belong to the superfamily of metallo-beta-lactamase fold proteins | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into vector pQTEV and introduced into Escherichia coli SCS1 cells carrying pRARE. The resulting clone used for expression of unlabeled protein. For expression of selenomethionine-labeled protein, the plasmid used to transform B834 Escherichia coli cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
at 1.7 A resolution, structure is organized into a four-layer alphabetabetaalpha topology, zinc ion residing between the central beta-sheets is partially coordinated by three histidine residues | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains a zinc-containing hydrolase fold | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration and on anion-exchange column | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl acetate + H2O | - |
Homo sapiens | nitrophenolate + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | in solution, crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PTD012 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
p-nitrophenyl acetate | - |
Homo sapiens |