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Literature summary for 3.4.17.1 extracted from

  • Manjasetty, B.A.; Buessow, K.; Fieber-Erdmann, M.; Roske, Y.; Gobom, J.; Scheich, C.; Goetz, F.; Niesen, F.H.; Heinemann, U.
    Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold (2006), Protein Sci., 15, 914-920.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information PTD012 might not belong to the carboxypeptidase family of enzymes, might belong to the superfamily of metallo-beta-lactamase fold proteins Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into vector pQTEV and introduced into Escherichia coli SCS1 cells carrying pRARE. The resulting clone used for expression of unlabeled protein. For expression of selenomethionine-labeled protein, the plasmid used to transform B834 Escherichia coli cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
at 1.7 A resolution, structure is organized into a four-layer alphabetabetaalpha topology, zinc ion residing between the central beta-sheets is partially coordinated by three histidine residues Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains a zinc-containing hydrolase fold Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration and on anion-exchange column Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl acetate + H2O
-
Homo sapiens nitrophenolate + acetate
-
?

Subunits

Subunits Comment Organism
monomer in solution, crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
PTD012
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
p-nitrophenyl acetate
-
Homo sapiens