Application | Comment | Organism |
---|---|---|
medicine | mutations that results in near undetectable activity of aspartocyclase correlate with Canavan Disease, a neurodegenerative disorder usually fatal during childhood | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
full-length human ASPA cDNA is subcloned into the pcDNA3.1/V5-His-TOPO vector, for bacterial expression mutants are generated directly in the pBAD/Thio-TOPO vector | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A305E | tested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
A57T | untested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
C124A | alanine substitution of Cys124, residue indicates by homology modelling to be in close proximity and in the proper orientation for disufide bonding | Homo sapiens |
C152A | alanine substitution of Cys152, residue indicates by homology modelling to be in close proximity and in the proper orientation for disufide bonding | Homo sapiens |
C152W | tested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
C61A | mutant for analyzing the biochemical basis for undetectable ASPA activity, and for testing of the hypothesis, that ASPA is a zinc-dependent metalloenzyme | Homo sapiens |
C61S | mutant for analyzing the biochemical basis for undetectable ASPA activity, and for testing of the hypothesis, that ASPA is a zinc-dependent metalloenzyme | Homo sapiens |
C61W | mutant for analyzing the biochemical basis for undetectable ASPA activity, and for testing of the hypothesis, that ASPA is a zinc-dependent metalloenzyme | Homo sapiens |
D204H | mutant for analyzing the biochemical basis for undetectable ASPA activity, and for testing of the hypothesis, that ASPA is a zinc-dependent metalloenzyme | Homo sapiens |
D249V | tested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
D68A | tested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
E178A | mutation of the general proton donor | Homo sapiens |
E214X | tested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
E24D | mutation of a putative zinc-binding residue | Homo sapiens |
E24G | mutation of a putative zinc-binding residue, tested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
E24H | mutant for analyzing the biochemical basis for undetectable ASPA activity, and for testing of the hypothesis, that ASPA is a zinc-dependent metalloenzyme | Homo sapiens |
E24H/H116E | mutant designed to switch the order of the zinc-binding residues | Homo sapiens |
E285A | tested Canavan Disease mutation | Homo sapiens |
F295S | untested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
G274R | untested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
H116E | mutant for analyzing the biochemical basis for undetectable ASPA activity, and for testing of the hypothesis, that ASPA is a zinc-dependent metalloenzyme | Homo sapiens |
H116G | mutation of a putative zinc-binding residue | Homo sapiens |
H21E/E24H | mutant designed to switch the order of the zinc-binding residues | Homo sapiens |
H21G | mutation of a putative zinc-binding residue | Homo sapiens |
H21P | untested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
I143T | untested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
K213E/G274R | untested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
M195R | untested Canavan Disease mutation, results in undetectable enzyme activity | Homo sapiens |
P183H | untested Canavan Disease mutation | Homo sapiens |
R63N | mutation that affects transition state stabilization | Homo sapiens |
R71N | mutation that affects substrate carboxyl binding | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
native ASPA expessed in COS-7 cells, determined by immunoblotting | Homo sapiens |
52000 | - |
recombinant human ASPA fused to N-terminal thioredoxin and C-terimal V5 and a 6x-histidine tag, analyzed by SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-aspartate | - |
Homo sapiens | acetate + L-aspartate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ASPA | - |
Homo sapiens |
aspartoacyclase | - |
Homo sapiens |
carboxypeptidase a | - |
Homo sapiens |