Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Trypsin | activates zymogen | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | engaged in prostate cancer | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
hCPA4 is produced as a zymogen through recombinant heterologous overexpression using vector pPIC9 and the methylotrophic yeast Pichia pastoris | Homo sapiens |
produced as a zymogen through recombinant heterologous overexpression using vector pPIC9 and the methylotrophic yeast Pichia pastoris as expression host | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
CPA4-hexapeptide complex crystallized by sitting drop vapor diffusion method, to 1.6 A resolution | Homo sapiens |
structural details of a true cleaved double-product complex with a hexapeptide of human ocarboxypeptidase A4 employing diffraction data to 1.6 A resolution are provided | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Gly-Asn-Arg-Pro-Thr | - |
Homo sapiens | |
Gly-Asn-Arg-Pro-Val-Thr | - |
Homo sapiens | |
Phe-Asn-Arg-Ala-Val-Asp | - |
Homo sapiens | |
Phe-Asn-Arg-Ala-Val-Val | - |
Homo sapiens | |
Phe-Asn-Arg-Pro-Val | - |
Homo sapiens | |
Phe-Asn-Arg-Pro-Val-Asp | - |
Homo sapiens | |
Phe-Asn-Arg-Pro-Val-Val | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UI42 | - |
- |
Purification (Comment) | Organism |
---|---|
by anion-exchange chromatography | Homo sapiens |
the active protein is obtained through tryptic activation, the resulting protein-product complex is subsequently purified by anion-exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
prostate cancer cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(4-methoxyphenyl-azoformyl)-L-Phe + H2O | - |
Homo sapiens | ? | - |
? | |
Phe-Asn-Arg-Pro-Val + H2O | used as substrate in the activity assay | Homo sapiens | ? | - |
? | |
Phe-Asn-Arg-Pro-Val-Asp + H2O | used as substrate in the activity assay | Homo sapiens | ? | - |
? | |
Phe-Asn-Arg-Pro-Val-Val + H2O | used as substrate in the activity assay | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
A-type metallocarboxypeptidase | - |
Homo sapiens |
carboxypeptidase a | - |
Homo sapiens |
carboxypeptidase A4 | - |
Homo sapiens |
CPA4 | - |
Homo sapiens |
hCPA4 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.63 | - |
- |
Homo sapiens | Phe-Asn-Arg-Ala-Val-Val | |
0.71 | - |
- |
Homo sapiens | Phe-Asn-Arg-Pro-Val-Val | |
0.72 | - |
- |
Homo sapiens | Phe-Asn-Arg-Ala-Val-Asp | |
0.91 | - |
- |
Homo sapiens | Phe-Asn-Arg-Pro-Val-Asp | |
2.5 | - |
- |
Homo sapiens | Gly-Asn-Arg-Pro-Thr | |
4 | - |
- |
Homo sapiens | Phe-Asn-Arg-Pro-Val | |
5 | - |
IC50 4-6 mM | Homo sapiens | Phe-Asn-Arg-Pro-Val |