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Literature summary for 3.4.17.1 extracted from

  • Pallares, I.; Bonet, R.; Garcia-Castellanos, R.; Ventura, S.; Aviles, F.X.; Vendrell, J.; Gomis-Rueth, F.X.
    Structure of human carboxypeptidase A4 with its endogenous protein inhibitor, latexin (2005), Proc. Natl. Acad. Sci. USA, 102, 3978-3983.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.17.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of CPA4 in Pichia pastoris, scecretion of the recombinant enzyme to the culture medium Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant CPA4 in complex with latexin, hanging drop vapour diffusion method, 0.001 ml of 7 mg/ml protein solution is mixed with 0.001 ml reservoir solution containing 40% 2-methyl-2,4-pentanediol, 0.1 M [bis(2-hydroxyethyl)amino]tris(hydroxymethyl)methane, pH 6.5, and with 0.002 ml 40% acetone, 20°C, X-ray diffraction structure determination and analysis at 1.6 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
latexin cloned from human brain and expressed in Escherichia coli, CPA4-latexin complex structure determination and analysis Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(methoxyphenyl-azoformyl)-Phe-OH + H2O
-
 Homo sapiens ?
-
 ?
N-[3-(2-furyl)acryloyl]-Phe-Phe + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
carboxypeptidase A4
-
 Homo sapiens
CPA4
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000003
-
 latexin pH 7.5, recombinant CPA4 Homo sapiens