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Literature summary for 3.4.17.1 extracted from
- Cloning and characterization of cDNAS encoding carboxypeptidase-like proteins from the gut of Hessian fly larvae Mayetiola destructor (Say) (2006), Insect Biochem. Mol. Biol., 36, 665-673.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a cDNA library is constructed using the SMART cDNA construction kit, the PCR fragments are cloned directly into plasmid pCR2.1-TOPO | Mayetiola destructor |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47570 | - |
theoretical molecular mass MDCP-A2 | Mayetiola destructor |
| 47790 | - |
theoretical molecular mass MDCP-A1d | Mayetiola destructor |
| 47820 | - |
theoretical molecular mass MDCP-A1a | Mayetiola destructor |
| 47850 | - |
theoretical molecular mass MDCP-A1e | Mayetiola destructor |
| 47890 | - |
theoretical molecular mass MDCP-A1b | Mayetiola destructor |
| 47890 | - |
theoretical molecular mass MDCP-A1c | Mayetiola destructor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mayetiola destructor | Q0QWG2 | MDCP-A2 | - |
| Mayetiola destructor | Q0QWG3 | MDCP-A1e | - |
| Mayetiola destructor | Q0QWG4 | MDCP-A1d | - |
| Mayetiola destructor | Q0QWG5 | MDCP-A1c | - |
| Mayetiola destructor | Q0QWG6 | MDCP-A1b | - |
| Mayetiola destructor | Q0QWG7 | MDCP-A1a | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| proteins were extracted from larvae and pupae of the insect, and adult flies | Mayetiola destructor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gut | - |
Mayetiola destructor | - |
| salivary gland | - |
Mayetiola destructor | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-(3-[2-furyl]acryloyl)-Phe-Phe + H2O | substrate of carboxypeptidase activity assay | Mayetiola destructor | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carboxypeptidase A1 | - |
Mayetiola destructor |
| carboxypeptidase A2 | - |
Mayetiola destructor |
| MDCP-A1 | - |
Mayetiola destructor |
| MDCP-A2 | - |
Mayetiola destructor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
carboxypeptidase activity assay | Mayetiola destructor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
carboxypeptidase activity assay | Mayetiola destructor |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mayetiola destructor | theoretical pI value MDCP-A1b | - |
5.15 |
| Mayetiola destructor | theoretical pI value MDCP-A1c | - |
5.15 |
| Mayetiola destructor | theoretical pI value MDCP-A1a | - |
5.22 |
| Mayetiola destructor | theoretical pI value MDCP-A1e | - |
5.22 |
| Mayetiola destructor | theoretical pI value MDCP-A1d | - |
5.29 |
| Mayetiola destructor | theoretical pI value MDCP-A2 | - |
6.46 |
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