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Literature summary for 3.4.17.1 extracted from

  • Cho, J.H.; Kim, D.H.; Lee, K.J.; Choi, K.Y.
    The role of Tyr248 probed by mutant bovine carboxypeptidase A: insight into the catalytic mechanism of carboxypeptidase A (2001), Biochemistry, 40, 10197-10203.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

Protein Variants

Protein Variants Comment Organism
Y248A the ratio of turnover number to Km-value with hippuryl-L-Phe as substrate is 113fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value with N-[3-(2-furyl)acryloyl]-Phe-Phe as substrate is 262fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value with O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate as substrate is 85fold lower than that of the wild-type enzyme. The Ki-value for (2R,3S)-2-benzyl-3,4-epoxybutanoic acid is 3.1fold higher than the wild-type value Bos taurus
Y248F the ratio of turnover number to Km-value with hippuryl-L-Phe as substrate is 49fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value with N-[3-(2-furyl)acryloyl]-Phe-Phe as substrate is 49fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value with O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate as substrate is 52.4fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value with hippuryl-DL-beta-phenyllactate as substrate is 6.5fold lower than that of the wild-type enzyme.The Ki-value for (2R,3S)-2-benzyl-3,4-epoxybutanoic acid is 86% of the wild-type value Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
(2R,3S)-2-benzyl-3,4-epoxybutanoic acid
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.49
-
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate pH 7.5, 25°C, mutant enzyme Y248A Bos taurus
3.3
-
hippuryl-L-Phe pH 7.5, 25°C, mutant enzyme Y248A Bos taurus
5.6
-
hippuryl-L-Phe pH 7.5, 25°C, mutant enzyme Y248F Bos taurus
5.8
-
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate pH 7.5, 25°C, mutant enzyme Y248F Bos taurus
27
-
N-[3-(2-furyl)acryloyl]-Phe-Phe pH 7.5, 25°C, mutant enzyme Y248A Bos taurus
60
-
hippuryl-L-Phe pH 7.5, 25°C, wild-type enzyme Bos taurus
76
-
N-[3-(2-furyl)acryloyl]-Phe-Phe pH 7.5, 25°C, mutant enzyme Y248F Bos taurus
120
-
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate pH 7.5, 25°C, wild-type enzyme Bos taurus
340
-
N-[3-(2-furyl)acryloyl]-Phe-Phe pH 7.5, 25°C, wild-type enzyme Bos taurus
780
-
hippuryl-DL-beta-phenyllactate pH 7.5, 25°C, mutant enzyme Y248F Bos taurus
1300
-
hippuryl-DL-beta-phenyllactate pH 7.5, 25°C, wild-type enzyme Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hippuryl-DL-beta-phenyllactate + H2O
-
Bos taurus ?
-
?
hippuryl-L-Phe + H2O
-
Bos taurus hippuric acid + L-Phe
-
?
N-[3-(2-furyl)acryloyl]-Phe-Phe + H2O
-
Bos taurus N-[3-(2-furyl)acryloyl]-Phe + Phe
-
?
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate + H2O
-
Bos taurus ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
N-[3-(2-furyl)acryloyl]-Phe-Phe pH 7.5, 25°C, wild-type enzyme Bos taurus
0.1
-
hippuryl-DL-beta-phenyllactate pH 7.5, 25°C, wild-type enzyme Bos taurus
0.11
-
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate pH 7.5, 25°C, wild-type enzyme Bos taurus
0.27
-
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate pH 7.5, 25°C, mutant enzyme Y248F Bos taurus
0.39
-
hippuryl-DL-beta-phenyllactate pH 7.5, 25°C, mutant enzyme Y248F Bos taurus
0.55
-
N-[3-(2-furyl)acryloyl]-Phe-Phe pH 7.5, 25°C, mutant enzyme Y248F Bos taurus
0.56
-
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate pH 7.5, 25°C, mutant enzyme Y248A Bos taurus
0.88
-
hippuryl-L-Phe pH 7.5, 25°C, wild-type enzyme Bos taurus
1
-
N-[3-(2-furyl)acryloyl]-Phe-Phe pH 7.5, 25°C, mutant enzyme Y248A Bos taurus
4
-
hippuryl-L-Phe pH 7.5, 25°C, mutant enzyme Y248F Bos taurus
5.1
-
hippuryl-L-Phe pH 7.5, 25°C, mutant enzyme Y248A Bos taurus
6.08
-
O-(trans-p-chlorocinnamoyl)-L-beta-phenyllactate pH 7.5, 25°C, mutant enzyme Y248A Bos taurus
6.08
-
hippuryl-L-Phe pH 7.5, 25°C, wild-type enzyme Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
(2R,3S)-2-benzyl-3,4-epoxybutanoic acid pH 7.5, 25°C, mutant enzyme Y248F Bos taurus
0.35
-
(2R,3S)-2-benzyl-3,4-epoxybutanoic acid pH 7.5, 25°C, wild-type enzyme Bos taurus
1.1
-
(2R,3S)-2-benzyl-3,4-epoxybutanoic acid pH 7.5, 25°C, mutant enzyme Y248A Bos taurus