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Literature summary for 3.4.17.1 extracted from
- Bovine procarboxypeptidase and carboxypeptidase A (1970), Methods Enzymol., 19, 460-503.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| carbobenzoxy-Gly-L-Phe | substrate inhibition | Bos taurus |  |
| additional information | substrate activation by some substrates | Bos taurus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Bos taurus |
General Stability
| General Stability | Organism |
|---|---|
| urea influences general stability, 4 M, 0°C, pH 7.5, 24 h, stable, 7 M, 0°C, pH 7.5, 24 h, unstable | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Phenylpropionic acid | Ki: 0.062 mM | Bos taurus | |
| 4-phenylbutyric acid | Ki: 0.118 mM | Bos taurus | |
| D-His | Ki: 20 mM | Bos taurus | |
| D-Phe | - |
Bos taurus | |
| Gly-L-Tyr | - |
Bos taurus | |
| indole acetic acid | Ki: 0.078 mM | Bos taurus | |
| p-nitrophenylacetic acid | Ki: 2.5 mM | Bos taurus | |
| phenylacetic acid | Ki: 0.39 mM | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | derivation from classical Michalis-Menten kinetics with some substrates | Bos taurus | |
| 0.05 | - |
N-trans-3-(-3-indoleacryloyl)-L-Phe | - |
Bos taurus | |
| 0.81 | 11 | hippuryl-L-Phe | - |
Bos taurus | |
| 1.95 | 37 | carbobenzoxy-Gly-L-Phe | - |
Bos taurus | |
| 6.1 | 7.2 | carbobenzoxy-Gly-L-Trp | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| secretory granule | exocytosed from | Bos taurus | 30141 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | metalloenzyme | Bos taurus | |
| Zn2+ | 0.98-1.03 mol Zn per mol enzyme | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
molecular weight of dimeric procarboxypeptidase 72500 | Bos taurus |
| additional information | - |
molecular weight of trimeric procarboxypeptidase 87000, molecular weight of carboxypeptidase precursor subunit 40000-42000, slight differences in molecular weight may be caused by different activation conditions | Bos taurus |
| 34490 | - |
amino acid sequence | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| also procarboxypeptidase | Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro | mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, lyophilized powder, indefinite, proenzyme | Bos taurus |
| 0°C, Tris-HCl, pH 7.5, crystals under toluene atmosphere, indefinite | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbobenzoxy-Gly-Gly-L-Tyr + H2O | - |
Bos taurus | carbobenzoxy-Gly-Gly + L-Tyr | - |
ir | |
| carbobenzoxy-Gly-L-Phe + H2O | - |
Bos taurus | carbobenzoxy-Gly + L-Phe | - |
ir | |
| carbobenzoxy-Gly-L-Trp + H2O | - |
Bos taurus | carbobenzoxy-Gly + L-Trp | - |
ir | |
| carbobenzyloxy-Gly-hippuryl-L-Phe + H2O | - |
Bos taurus | carbobenzyloxy-Gly-hippuric acid + L-Phe | - |
ir | |
| hippuryl-L-Phe + H2O | - |
Bos taurus | hippuric acid + L-Phe | - |
ir | |
| N-(trans-3-indoleacryloyl)-L-Phe + H2O | - |
Bos taurus | trans-3-indoleacrylate + L-Phe | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | model of three-dimensional structure | Bos taurus |
| More | proenzyme exists as aggregates of 2-3 subunits | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bos taurus |
| 37 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 73.4 | 90.5 | carbobenzoxy-Gly-L-Trp | - |
Bos taurus | |
| 85 | - |
carbobenzoxy-Gly-Gly-L-Tyr | - |
Bos taurus | |
| 90 | 198 | carbobenzoxy-Gly-L-Phe | - |
Bos taurus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
| 7 | 10 | at 0°C | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.062 | - |
3-Phenylpropionic acid | - |
Bos taurus | |
| 0.078 | - |
indole acetic acid | - |
Bos taurus | |
| 0.118 | - |
4-phenylbutyric acid | - |
Bos taurus | |
| 0.39 | - |
phenylacetic acid | - |
Bos taurus | |
| 2.5 | - |
p-nitrophenylacetic acid | - |
Bos taurus | |
| 20 | - |
D-His | - |
Bos taurus | |
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