Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.13.9 extracted from

  • Zhou, L.; Yuan, J.; Yin, J.; Wang, E.
    Kinetic study of prolidase activity in erythrocytes against different substrates using capillary electrophoresis with electrochemiluminescence detection (2008), J. Chromatogr. A, 1200, 255-259.
    View publication on PubMed
Search for more literature for 3.4.13.9

Activating Compound

Activating Compound Comment Organism Structure
Colchicine colchicine enhances PLD activity for Gly-L-Pro and L-Leu-L-Pro, the highest enhancement of PLD on Gly-L-Pro is 141% at 0.01 mM Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Colchicine colchicine has a slight inhibiting effect on prolidase activity for L-Val-L-Pro, the activity reaches its highest inhibition percentage of 59% at 2.0 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.243
-
 L-Val-L-Pro in the presence of 0.1 mM colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C Homo sapiens
0.269
-
 L-Val-L-Pro in the absence of colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C Homo sapiens
0.395
-
 L-Leu-L-Pro in the presence of 0.1 mM colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C Homo sapiens
0.628
-
 L-Leu-L-Pro in the absence of colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C Homo sapiens
2.13
-
 Gly-L-Pro in the presence of 0.1 mM colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C Homo sapiens
6.23
-
 Gly-L-Pro in the absence of colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required for activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-L-Pro + H2O
-
 Homo sapiens Gly + L-Pro
-
 ?
L-Leu-L-Pro + H2O
-
 Homo sapiens L-Leu + L-Pro
-
 ?
L-Val-L-Pro + H2O
-
 Homo sapiens L-Val + L-Pro
-
 ?

Synonyms

Synonyms Comment Organism
PLD
-
 Homo sapiens
prolidase
-
 Homo sapiens