Inhibitors | Comment | Organism | Structure |
---|---|---|---|
captopril | inhibits isozyme PD I dose-dependently, but shows no inhibition of isozyme PD II at 0.1 mM | Rattus norvegicus | |
N-Benzyloxycarbonyl-L-proline | inhibits isozyme PD I dose-dependently, but shows no inhibition of isozyme PD II at up to 1 mM | Rattus norvegicus | |
Ni2+ | - |
Rattus norvegicus | |
NiCl2 | inhibits isozye PD II much more effectively than isozyme PD I | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | lack of prolidase I leads to prolidase deficiency, a disease characterized by intractable skin lesions, recurrent respiratory infections, and mental retardation, physiologic roles of prolidase isoenzymes, PD I functions by way of an intestinal peptide carrier, which may be regulated by the uptake of various iminodipeptides, intestinal PD II also participates in absorption of proline and other amino acids early in life, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar rats, isozymes PD I and PD II | - |
Purification (Comment) | Organism |
---|---|
native isozymes partially from kidney by anion exchange chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | isozymes PD I and PD II, high expression level of isozyme PD I, which is predominantly concentrated in renal cortex, where its activity is 2fold higher than in renal medulla | Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
additional information | organ distribution and developmental changes of isozymes PD I and PD II, overview | Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
small intestine | high expression level of isozyme PD I | Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
stomach | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro + H2O | preferred substrate of isozyme PD I | Rattus norvegicus | Ala + Pro | - |
? | |
Gly-Pro + H2O | - |
Rattus norvegicus | Gly + Pro | - |
? | |
Met-Pro + H2O | preferred substrate of isozyme PD II | Rattus norvegicus | Met + Pro | - |
? | |
additional information | substrate specificity of isozymes, overview | Rattus norvegicus | ? | - |
? | |
additional information | lack of prolidase I leads to prolidase deficiency, a disease characterized by intractable skin lesions, recurrent respiratory infections, and mental retardation, physiologic roles of prolidase isoenzymes, PD I functions by way of an intestinal peptide carrier, which may be regulated by the uptake of various iminodipeptides, intestinal PD II also participates in absorption of proline and other amino acids early in life, overview | Rattus norvegicus | ? | - |
? | |
Ser-Pro + H2O | preferred substrate of isozyme PD I | Rattus norvegicus | Ser + Pro | - |
? | |
Val-Pro + H2O | - |
Rattus norvegicus | Val + Pro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
prolidase I | - |
Rattus norvegicus |
prolidase II | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.005 | - |
isozyme PD I, pH 7.8, 37°C | Rattus norvegicus | N-Benzyloxycarbonyl-L-proline | |
0.05 | - |
isozyme PD I, pH 7.8, 37°C | Rattus norvegicus | captopril | |
0.05 | - |
isozyme PD II, pH 7.8, 37°C | Rattus norvegicus | Ni2+ |