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Literature summary for 3.4.13.9 extracted from

  • Du, X.; Tove, S.; Kast-Hutcheson, K.; Grunden, A.M.
    Characterization of the dinuclear metal center of Pyrococcus furiosus prolidase by analysis of targeted mutants (2005), FEBS Lett., 579, 6140-6146.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D209A less than 0.1% enzymic activity, contains 0.7 Co per subunit, maximal activity with 0.5 mM Co2+, less than 20% residual activity with 10 mM Co2+ Pyrococcus furiosus
E313L protein is highly misfolded, remains aggregated and recalcitrant during purification Pyrococcus furiosus
E327L no enzymic activity, contains 0.03 Co per sunbunit Pyrococcus furiosus
H284A less than 0.1% enzymic activity, protein is unfolded Pyrococcus furiosus
H284L less than 0.1% enzymic activity, contains 0.28 Co per subunit. Inhibitory effect of high Co2+-concentration is less pronounced than in wild-type Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ one Co-bound dinuclear metal cluster per monomer, Co1 is the tight binding, Co2 the loose binding metal center. Wild-type exhibits maximal activity in presence of 0.5 mM Co2+, less than 20% residual activity with 10 mM Co2+ Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.67
-
mutant H284A, pH 7.0, 100°C Pyrococcus furiosus
0.73
-
mutant H284L, pH 7.0, 100°C Pyrococcus furiosus
1.02
-
mutant D209A, pH 7.0, 100°C Pyrococcus furiosus
1388
-
wild-type, pH 7.0, 100°C Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Met-L-Pro
-
Pyrococcus furiosus L-Met + L-Pro
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
Pyrococcus furiosus