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Literature summary for 3.4.13.9 extracted from
- Purification and characterization of recombinant human liver prolidase expressed in Saccharomyces cerevisiae (2005), Arch. Toxicol., 79, 253-259.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 55% residual activity | Homo sapiens |  |
| Cu2+ | strong | Homo sapiens | |
| EDTA | 1 mM, 70% residual activity | Homo sapiens | |
| Zn2+ | strong | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | stimulation | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
expression in Saccharomyces cerevisiae, bifunctional enzyme with both dipeptidase and organophosphorus acid anhydrolase activity | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, expression in Saccharomyces cerevisiae | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.632 | - |
37°C, pH 7.8, dipeptidase activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gly-L-Pro + H2O | - |
Homo sapiens | Gly + L-Pro | - |
? | |
| Met-Pro + H2O | 68% of the activity with Gly-L-Pro | Homo sapiens | L-Met + L-Pro | - |
? | |
| soman + H2O | organophosphorus acid anhydrolase activity | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
dipeptidase activity, sharp decrease in activity above | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
dipeptidase activity, 20% of maximum activity | Homo sapiens |
| 8 | - |
dipeptidase activity | Homo sapiens |
| 10 | - |
dipeptidase activity, 80% of maximum activity | Homo sapiens |
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Release 2023.1 (January 2023)
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