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Literature summary for 3.4.11.9 extracted from
- Unveiling aminopeptidase P from Streptomyces lavendulae: molecular cloning, expression and biochemical characterization (2014), Enzyme Microb. Technol., 55, 7-13.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Streptomyces lavendulae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Streptomyces lavendulae |  |
| DTT | - |
Streptomyces lavendulae | |
| EDTA | - |
Streptomyces lavendulae | |
| PMSF | - |
Streptomyces lavendulae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4697 | - |
Gly-Pro-4-nitroanilide | pH 8, 37°C | Streptomyces lavendulae | |
| 0.6396 | - |
bradykinin | pH 8, 37°C | Streptomyces lavendulae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | enhances activity | Streptomyces lavendulae | |
| Cu2+ | enhances activity | Streptomyces lavendulae | |
| Mg2+ | enhances activity | Streptomyces lavendulae | |
| Mn2+ | enhances activity, atomic absorption studies reveal the presence of Mn2+ in the protein as a co-factor | Streptomyces lavendulae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
SDS-PAGE | Streptomyces lavendulae |
| 240000 | - |
native PAGE | Streptomyces lavendulae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces lavendulae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Streptomyces lavendulae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bradykinin + H2O | - |
Streptomyces lavendulae | ? | - |
? | |
| Gly-Pro-4-nitroanilide + H2O | - |
Streptomyces lavendulae | Gly + Pro-4-nitroanilide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | SDS-PAGE, 4* 60000 | Streptomyces lavendulae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase P | - |
Streptomyces lavendulae |
| APP | - |
Streptomyces lavendulae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
enzyme retains 50% of activity after 30 min of incubation | Streptomyces lavendulae |
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