Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.9 extracted from

  • Hauser, F.; Strassner, J.; Schaller, A.
    Cloning, expression, and characterization of tomato (Lycopersicon esculentum) aminopeptidase P (2001), J. Biol. Chem., 276, 31732-31737.
    View publication on PubMed
Search for more literature for 3.4.11.9

Cloned(Commentary)

Cloned (Comment) Organism
gene LeAPP1, DNA and amino acid sequence determination and analysis Solanum lycopersicum
gene LeAPP2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli in N-terminal fusion with GST, a mutant form lacking 11 amino acids from the N-terminus is not active Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 50% inhibition at 0.036 mM Solanum lycopersicum
2-mercaptoethanol 50% inhibition at 0.043 mM Solanum lycopersicum
Ca2+ stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM Solanum lycopersicum
Co2+ stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM Solanum lycopersicum
Cu2+ complete inhibition at 4 mM Solanum lycopersicum
diethyldicarbonate 50% inhibition at 0.011 mM Solanum lycopersicum
EDTA 50% inhibition at 0.34 m Solanum lycopersicum
NEM 50% inhibition at 0.079 mM Solanum lycopersicum
Zn2+ complete inhibition at 4 mM Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Solanum lycopersicum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM Solanum lycopersicum
Co2+ stimulation of enzyme activity at 0.4 mM, inhibition at 4 mM Solanum lycopersicum
Mg2+ stimulation of the enzyme Solanum lycopersicum
Mn2+ stimulation of the enzyme, most effective at 4 mM Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 x * 105000, recombinant GST-fusion protein, SDS-PAGE Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum Q93X45 gene LeAPP2, isozyme APP2
-
Solanum lycopersicum Q93X46 gene LeAPP1, isozyme APP1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme APP2 from Escherichia coli Solanum lycopersicum

Reaction

Reaction Comment Organism Reaction ID
release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide isozyme APP-2 shows a preference for Arg-Pro-Pro-, Arg-Pro-Lys-, Pro-Pro-Gly-, -Phe-Gly- in descending order Solanum lycopersicum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Solanum lycopersicum

Storage Stability

Storage Stability Organism
-20°C to 4°C, 0.5 mg/ml recombinant GST-fusion enzyme, several weeks without significant loss of activity Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bradykinin + H2O i.e. RPPGFSPFR, rapid hydrolysis of the N-terminal Arg1-Pro2 bond Solanum lycopersicum Arg + PPGFSPFR
-
 ?
additional information systemin, a peptide hormone-like signaling molecule from tomato plants, is no substrate Solanum lycopersicum ?
-
 ?
Nalpha-(2-aminobenzoyl)-Lys-Pro-Pro 4-nitroanilide + H2O
-
 Solanum lycopersicum Nalpha-(2-aminobenzoyl)-Lys + Pro-Pro-4-nitroanilide
-
 ?
PPGFSPFR + H2O low activity Solanum lycopersicum Pro + PGFSPFR
-
 ?
substance P + H2O i.e. RPKPQQFFGLM, hydrolysis of the N-terminal Arg1-Pro2 bond Solanum lycopersicum Arg + PKPQQFFGLM
-
 ?

Subunits

Subunits Comment Organism
? x * 105000, recombinant GST-fusion protein, SDS-PAGE Solanum lycopersicum

Synonyms

Synonyms Comment Organism
aminopeptidase P
-
 Solanum lycopersicum
LeAPP1
-
 Solanum lycopersicum
LeAPP2
-
 Solanum lycopersicum
More the enzyme belongs to the peptidase family M24 Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Solanum lycopersicum