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Literature summary for 3.4.11.9 extracted from
- A novel assay method for aminopeptidase P and partial purification of two types of the enzyme in Escherichia coli (1988), Agric. Biol. Chem., 52, 1957-1963.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Escherichia coli |  |
| PCMB | partial | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 0.05 mM, 3-4fold activatiion | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 210000 | - |
enzyme form APP-II, gel filtration | Escherichia coli |
| 350000 | - |
enzyme form APP-I, gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
HB101, 2 types of enzyme: APP-I and APP-II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.21 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gly-Pro-2-naphthylamide + H2O | - |
Escherichia coli | Gly + Pro-2-naphthylamide | - |
? | |
| Gly-Pro-4-methylcoumarin 7-amide + H2O | - |
Escherichia coli | Gly + Pro-4-methylcoumarin 7-amide | - |
? | |
| Gly-Pro-p-nitroanilide + H2O | - |
Escherichia coli | Gly + Pro-p-nitroanilide | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 41 | - |
enzyme form APP-II | Escherichia coli |
| 55 | - |
enzyme form APP-I | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 8.0, 30 min, 50% loss of activity of enzyme form APP-I | Escherichia coli |
| 60 | - |
pH 8.0, 30 min, 50% loss of activity of enzyme form APP-II | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
enzyme form APP-I | Escherichia coli |
| 9 | - |
enzyme form APP-II | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
50°C, 30 min, 50% loss of activity of enzyme form APP-I, 60°C, 50% loss of activity of enzyme form APP-II | Escherichia coli |
| 8 | 9 | stable, enzyme form APP-II | Escherichia coli |
| 10 | - |
stable, enzyme form APP-I | Escherichia coli |
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