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Literature summary for 3.4.11.6 extracted from

  • Hwang, S.R.; ONeill, A.; Bark, S.; Foulon, T.; Hook, V.
    Secretory vesicle aminopeptidase B related to neuropeptide processing: molecular identification and subcellular localization to enkephalin- and NPY-containing chromaffin granules (2007), J. Neurochem., 100, 1340-1350.
    View publication on PubMed
Search for more literature for 3.4.11.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rattus norvegicus
expressed in Escherichia coli Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
arphamenine A complete inhibition at 0.1 mM Bos taurus
arphamenine A complete inhibition at 0.1 mM Rattus norvegicus
bestatin complete inhibition at 0.1 mM Bos taurus
bestatin complete inhibition at 0.1 mM Rattus norvegicus
beta-mercaptoethanol 4% inhibition at 10 mM Bos taurus
beta-mercaptoethanol 20% inhibition at 10 mM Rattus norvegicus
Co2+ 62% inhibition at 1 mM Bos taurus
Co2+ 45% inhibition at 1 mM Rattus norvegicus
cysteine 21% inhibition at 1 mM Bos taurus
cysteine 15% inhibition at 1 mM Rattus norvegicus
dithiothreitol 33% inhibition at 10 mM Bos taurus
dithiothreitol 54% inhibition at 10 mM Rattus norvegicus
E64 48% inhibition at 0.01 mM Bos taurus
E64 12% inhibition at 0.01 mM Rattus norvegicus
EDTA 12% inhibition at 1 mM Bos taurus
EDTA 54% inhibition at 1 mM Rattus norvegicus
N-ethylmaleimide 44% inhibition at 0.1 mM Bos taurus
N-ethylmaleimide 21% inhibition at 0.1 mM Rattus norvegicus
Ni2+ 75% inhibition at 1 mM Bos taurus
Ni2+ 63% inhibition at 1 mM Rattus norvegicus
Phenylmethanesulfonylfluoride 10% inhibition at 0.1 mM Bos taurus
Phenylmethanesulfonylfluoride 1% inhibition at 0.1 mM Rattus norvegicus
puromycin 10% inhibition at 0.1 mM Bos taurus
puromycin 1% inhibition at 0.1 mM Rattus norvegicus
Zn2+ complete inhibition at 0.1 mM Bos taurus
Zn2+ 99% inhibition at 0.1 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 L-Arg-7-amido-4-methylcoumarin
-
 Rattus norvegicus
0.091
-
 L-Arg-7-amido-4-methylcoumarin
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
secretory vesicle present in secretory vesicles that contain cathepsin L with the neuropeptides enkephalin and neuropeptide Y Rattus norvegicus 99503
-
secretory vesicle present in secretory vesicles that contain cathepsin L with the neuropeptides enkephalin and neuropeptide Y Bos taurus 99503
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ 110% increase of activity at 150 mM Rattus norvegicus
Na+ 380% increase of activity at 150 mM Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72100
-
 calculated from amino acid sequence Bos taurus
74000
-
 SDS-PAGE Rattus norvegicus
74000
-
 SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus A2T1U6
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal medulla
-
 Rattus norvegicus
-
adrenal medulla
-
 Bos taurus
-
brain
-
 Rattus norvegicus
-
hypothalamus
-
 Rattus norvegicus
-
pancreas
-
 Rattus norvegicus
-
pituitary gland
-
 Rattus norvegicus
-
pituitary gland
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Arg-(Met)enkephalin + H2O
-
 Rattus norvegicus L-Arg + (Met)enkephalin
-
 ?
L-Arg-(Met)enkephalin + H2O
-
 Bos taurus L-Arg + (Met)enkephalin
-
 ?
L-Arg-7-amido-4-methylcoumarin + H2O 100% activity Rattus norvegicus L-Arg + 7-amino-4-methylcoumarin
-
 ?
L-Arg-7-amido-4-methylcoumarin + H2O 100% activity Bos taurus L-Arg + 7-amino-4-methylcoumarin
-
 ?
L-Asn-7-amido-4-methylcoumarin + H2O 53% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Asn + 7-amino-4-methylcoumarin
-
 ?
L-Asp-7-amido-4-methylcoumarin + H2O 15% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Asp + 7-amino-4-methylcoumarin
-
 ?
L-Cys-7-amido-4-methylcoumarin + H2O 5% activity compared to L-Arg-7-amido-4-methylcoumarin Rattus norvegicus L-Cys + 7-amino-4-methylcoumarin
-
 ?
L-Leu-7-amido-4-methylcoumarin + H2O 28% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Leu + 7-amino-4-methylcoumarin
-
 ?
L-Lys-(Met)enkephalin + H2O
-
 Rattus norvegicus L-Lys + (Met)enkephalin
-
 ?
L-Lys-(Met)enkephalin + H2O
-
 Bos taurus L-Lys + (Met)enkephalin
-
 ?
L-Lys-7-amido-4-methylcoumarin + H2O 10% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Lys + 7-amino-4-methylcoumarin
-
 ?
L-Lys-7-amido-4-methylcoumarin + H2O 17% activity compared to L-Arg-7-amido-4-methylcoumarin Rattus norvegicus L-Lys + 7-amino-4-methylcoumarin
-
 ?
L-Met-7-amido-4-methylcoumarin + H2O 19% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Met + 7-amino-4-methylcoumarin
-
 ?
L-Phe-7-amido-4-methylcoumarin + H2O 2% activity compared to L-Arg-7-amido-4-methylcoumarin Rattus norvegicus L-Phe + 7-amino-4-methylcoumarin
-
 ?
L-Pro-7-amido-4-methylcoumarin + H2O 7% activity compared to L-Arg-7-amido-4-methylcoumarin Rattus norvegicus L-Pro + 7-amino-4-methylcoumarin
-
 ?
L-Ser-7-amido-4-methylcoumarin + H2O 15% activity compared to L-Arg-7-amido-4-methylcoumarin Bos taurus L-Ser + 7-amino-4-methylcoumarin
-
 ?
additional information no activity with L-Ala-7-amido-4-methylcoumarin, L-Asn-7-amido-4-methylcoumarin, L-Gln-7-amido-4-methylcoumarin, L-Glu-7-amido-4-methylcoumarin, Gly-7-amido-4-methylcoumarin, L-His-7-amido-4-methylcoumarin, L-Ile-7-amido-4-methylcoumarin, L-Leu-7-amido-4-methylcoumarin, L-Asp-7-amido-4-methylcoumarin, L-Thr-7-amido-4-methylcoumarin, L-Met-7-amido-4-methylcoumarin, L-Trp-7-amido-4-methylcoumarin, L-Ser-7-amido-4-methylcoumarin, L-Tyr-7-amido-4-methylcoumarin, and L-Val-7-amido-4-methylcoumarin Rattus norvegicus ?
-
 ?
additional information no activity with L-Ala-7-amido-4-methylcoumarin, L-Cys-7-amido-4-methylcoumarin, L-Gln-7-amido-4-methylcoumarin, L-Glu-7-amido-4-methylcoumarin, Gly-7-amido-4-methylcoumarin, L-His-7-amido-4-methylcoumarin, L-Ile-7-amido-4-methylcoumarin, L-Phe-7-amido-4-methylcoumarin, L-Pro-7-amido-4-methylcoumarin, L-Thr-7-amido-4-methylcoumarin, L-Trp-7-amido-4-methylcoumarin, L-Tyr-7-amido-4-methylcoumarin, and L-Val-7-amido-4-methylcoumarin Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
Ap-B
-
 Rattus norvegicus
Ap-B
-
 Bos taurus
Arg/Lys aminopeptidase
-
 Rattus norvegicus
Arg/Lys aminopeptidase
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5
-
 Rattus norvegicus
5.5 6.5
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5 7
-
 Rattus norvegicus
5 7
-
 Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus calculated from amino acid sequence
-
 5.3