Application | Comment | Organism |
---|---|---|
medicine | enzyme is immunogenic in humans. Use as a diagnostic antigen in an ELISA using sera from patiens with acute and chronic brucellosis. The assay shows 100% specificity and 100% sensitivity and no cross-reaction to crude extracts from Rhizobium tropici, Ochrobactrum anthropi, Yersinia enterocolitica, or Escherichia coli | Brucella melitensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Brucella melitensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.01 mM, complete inhibition | Brucella melitensis | |
bestatin | - |
Brucella melitensis | |
Ca2+ | slight inhibition | Brucella melitensis | |
Co2+ | slight inhibition | Brucella melitensis | |
E64 | slight inhibition | Brucella melitensis | |
EDTA | 2.5 mM, complete inhibition | Brucella melitensis | |
Hg2+ | 1 mM, complete inhibition | Brucella melitensis | |
Mg2+ | slight inhibition | Brucella melitensis | |
additional information | not inhibitory: leupeptin | Brucella melitensis | |
pepstatin | slight inhibition | Brucella melitensis | |
phenylmethylsulfonyl fluoride | slight inhibition | Brucella melitensis | |
Zn2+ | 1 mM, complete inhibition | Brucella melitensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Brucella melitensis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
gel filtration | Brucella melitensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella melitensis | Q8YG38 | isoform PepN | - |
Brucella melitensis 16M | Q8YG38 | isoform PepN | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150 | - |
- |
Brucella melitensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-4-nitroanilide + H2O | hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly | Brucella melitensis | Gly + 4-nitroaniline | - |
? | |
Gly-4-nitroanilide + H2O | hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly | Brucella melitensis 16M | Gly + 4-nitroaniline | - |
? | |
L-Ala-4-nitroanilide + H2O | hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly | Brucella melitensis | L-Ala + 4-nitroaniline | - |
? | |
L-Ala-4-nitroanilide + H2O | hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly | Brucella melitensis 16M | L-Ala + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly | Brucella melitensis | L-Leu + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly | Brucella melitensis 16M | L-Leu + 4-nitroaniline | - |
? | |
L-Lys-4-nitroanilide + H2O | hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly | Brucella melitensis | L-Lys + 4-nitroaniline | - |
? | |
L-Lys-4-nitroanilide + H2O | hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly | Brucella melitensis 16M | L-Lys + 4-nitroaniline | - |
? | |
L-Met-4-nitroanilide + H2O | hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly | Brucella melitensis | L-Met + 4-nitroaniline | - |
? | |
L-Met-4-nitroanilide + H2O | hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly | Brucella melitensis 16M | L-Met + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 98000, SDS-PAGE | Brucella melitensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Brucella melitensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | stable within this range, complete loss of activity above or below | Brucella melitensis |