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Literature summary for 3.4.11.2 extracted from

  • Contreras-Rodriguez, A.; Seleem, M.N.; Schurig, G.G.; Sriranganathan, N.; Boyle, S.M.; Lopez-Merino, A.
    Cloning, expression and characterization of immunogenic aminopeptidase N from Brucella melitensis (2006), FEMS Immunol. Med. Microbiol., 48, 252-256.
    View publication on PubMed

Application

Application Comment Organism
medicine enzyme is immunogenic in humans. Use as a diagnostic antigen in an ELISA using sera from patiens with acute and chronic brucellosis. The assay shows 100% specificity and 100% sensitivity and no cross-reaction to crude extracts from Rhizobium tropici, Ochrobactrum anthropi, Yersinia enterocolitica, or Escherichia coli Brucella melitensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Brucella melitensis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.01 mM, complete inhibition Brucella melitensis
bestatin
-
Brucella melitensis
Ca2+ slight inhibition Brucella melitensis
Co2+ slight inhibition Brucella melitensis
E64 slight inhibition Brucella melitensis
EDTA 2.5 mM, complete inhibition Brucella melitensis
Hg2+ 1 mM, complete inhibition Brucella melitensis
Mg2+ slight inhibition Brucella melitensis
additional information not inhibitory: leupeptin Brucella melitensis
pepstatin slight inhibition Brucella melitensis
phenylmethylsulfonyl fluoride slight inhibition Brucella melitensis
Zn2+ 1 mM, complete inhibition Brucella melitensis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Brucella melitensis 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
gel filtration Brucella melitensis

Organism

Organism UniProt Comment Textmining
Brucella melitensis Q8YG38 isoform PepN
-
Brucella melitensis 16M Q8YG38 isoform PepN
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
150
-
-
Brucella melitensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-4-nitroanilide + H2O hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly Brucella melitensis Gly + 4-nitroaniline
-
?
Gly-4-nitroanilide + H2O hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly Brucella melitensis 16M Gly + 4-nitroaniline
-
?
L-Ala-4-nitroanilide + H2O hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly Brucella melitensis L-Ala + 4-nitroaniline
-
?
L-Ala-4-nitroanilide + H2O hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly Brucella melitensis 16M L-Ala + 4-nitroaniline
-
?
L-Leu-4-nitroanilide + H2O hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly Brucella melitensis L-Leu + 4-nitroaniline
-
?
L-Leu-4-nitroanilide + H2O hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly Brucella melitensis 16M L-Leu + 4-nitroaniline
-
?
L-Lys-4-nitroanilide + H2O hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly Brucella melitensis L-Lys + 4-nitroaniline
-
?
L-Lys-4-nitroanilide + H2O hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly Brucella melitensis 16M L-Lys + 4-nitroaniline
-
?
L-Met-4-nitroanilide + H2O hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly Brucella melitensis L-Met + 4-nitroaniline
-
?
L-Met-4-nitroanilide + H2O hydrolytic activity on 4-nitroanilides in decreasing order: L-Ala, L-Lys, L-Leu, L-Met, L-Gly Brucella melitensis 16M L-Met + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
monomer 1 * 98000, SDS-PAGE Brucella melitensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Brucella melitensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5 stable within this range, complete loss of activity above or below Brucella melitensis