Literature summary for 3.4.11.2 extracted from

Barth, A.; Siekel, P.; Sedlarova, E.; Valent, A.; Tokhtaeva, E.
Study of immobilized and extracellular aminopeptidases of watermelon (2005), Acta Histochem., 107, 253-259.

Search for more literature for 3.4.11.2

Protein Variants

Protein Variants	Comment	Organism
additional information	immobilization of the extracellular enzyme by pectate and/or alginate on spherical particles of 4 mm diameter, method overview	Citrullus lanatus subsp. vulgaris

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Citrullus lanatus subsp. vulgaris	-	-

Organism

Organism	UniProt	Comment	Textmining
Citrullus lanatus subsp. vulgaris	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell suspension culture	long-term, from seedlings	Citrullus lanatus subsp. vulgaris	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.019	-	immobilized enzyme, substrate L-Phe-4-nitroanilide	Citrullus lanatus subsp. vulgaris
0.021	-	immobilized enzyme, substrate L-Tyr-4-nitroanilide	Citrullus lanatus subsp. vulgaris
0.023	-	immobilized enzyme, substrates L-Ala-4-nitroanilide or L-Arg-4-nitroanilide	Citrullus lanatus subsp. vulgaris
0.12	-	free enzyme, substrate L-Phe-4-nitroanilide	Citrullus lanatus subsp. vulgaris
0.15	-	free enzyme, substrate L-Tyr-4-nitroanilide	Citrullus lanatus subsp. vulgaris
0.18	-	free enzyme, substrate L-Arg-4-nitroanilide	Citrullus lanatus subsp. vulgaris
0.19	-	free enzyme, substrate L-Ala-4-nitroanilide	Citrullus lanatus subsp. vulgaris
0.36	-	free enzyme in presence of Tween 80, substrate L-Phe-4-nitroanilide	Citrullus lanatus subsp. vulgaris
0.46	-	free enzyme in presence of Tween 80, substrate L-Tyr-4-nitroanilide	Citrullus lanatus subsp. vulgaris
0.55	-	free enzyme in presence of Tween 80, substrate L-Arg-4-nitroanilide	Citrullus lanatus subsp. vulgaris
0.57	-	free enzyme in presence of Tween 80, substrate L-Ala-4-nitroanilide	Citrullus lanatus subsp. vulgaris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ala-2-naphthylamide + H2O	best substrate	Citrullus lanatus subsp. vulgaris	L-Ala + 2-naphthylamine	-	?
L-Ala-4-nitroanilide + H2O	best substrate	Citrullus lanatus subsp. vulgaris	L-Ala + 4-nitroaniline	-	?
L-Arg-2-naphthylamide + H2O	-	Citrullus lanatus subsp. vulgaris	L-Arg + 2-naphthylamine	-	?
L-Arg-4-nitroanilide + H2O	-	Citrullus lanatus subsp. vulgaris	L-Arg + 4-nitroaniline	-	?
L-Phe-2-naphthylamide + H2O	-	Citrullus lanatus subsp. vulgaris	L-Phe + 2-naphthylamine	-	?
L-Phe-4-nitroanilide + H2O	-	Citrullus lanatus subsp. vulgaris	L-Phe + 4-nitroaniline	-	?
L-Tyr-2-naphthylamide + H2O	-	Citrullus lanatus subsp. vulgaris	L-Tyr + 2-naphthylamine	-	?
L-Tyr-4-nitroanilide + H2O	-	Citrullus lanatus subsp. vulgaris	L-Tyr + 4-nitroaniline	-	?

Synonyms

Synonyms	Comment	Organism
extracellular aminopeptidase	-	Citrullus lanatus subsp. vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Citrullus lanatus subsp. vulgaris

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Release 2023.1 (January 2023)