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Literature summary for 3.4.11.2 extracted from

  • Midwinter R.G.; Pritchard, G.G.
    Aminopeptidase N from Streptococcus salivarius subsp. thermophilus NCDO 573: purification and properties (1994), J. Appl. Bacteriol., 77, 288-295.
    View publication on PubMed
Search for more literature for 3.4.11.2

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 100% inhibition at 1 mM Streptococcus salivarius
4-chloromercuribenzoate 100% inhibition at 1 mM Streptococcus salivarius
Co2+ 68% inhibition at 0.2 mM Streptococcus salivarius
Cu2+ 100% inhibition at 0.2 mM Streptococcus salivarius
EDTA 68% inhibition at 1 mM Streptococcus salivarius
iodoacetic acid 83% inhibition at 1 mM Streptococcus salivarius
Mg2+ not inhibitory at 0.2 mM Streptococcus salivarius
Mn2+ not inhibitory at 0.2 mM Streptococcus salivarius
Zn2+ 73% inhibition at 0.2 mM Streptococcus salivarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 Leu-4-methylcoumarin 7-amide
-
 Streptococcus salivarius
0.06
-
 Lys-4-methylcoumarin 7-amide
-
 Streptococcus salivarius
0.09
-
 Arg-4-nitroanilide
-
 Streptococcus salivarius
0.11
-
 Phe-4-nitroanilide
-
 Streptococcus salivarius
0.17
-
 L-Leu-4-nitroanilide
-
 Streptococcus salivarius
0.2
-
 L-Lys-4-nitroanilide
-
 Streptococcus salivarius
1.4
-
 L-Ala-4-nitroanilide
-
 Streptococcus salivarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
 SDS-PAGE Streptococcus salivarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aminoacyl peptide Streptococcus salivarius
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus salivarius
-
 subsp. thermophilus NCDO 573
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus salivarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6 15 aminoacyl-4-nitroanilides as substrates Streptococcus salivarius
4
-
 Lys-4-methylcoumarin 7-amide as substrate Streptococcus salivarius
5.7
-
 Leu-4-methylcoumarin 7-amide as substrate Streptococcus salivarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aminoacyl peptide
-
 Streptococcus salivarius ?
-
 ?
aminoacyl-p-nitroanilide + H2O
-
 Streptococcus salivarius amino acid + nitroaniline
-
 ?
dipeptides + H2O
-
 Streptococcus salivarius amino acids
-
 ?
L-Ala-4-nitroanilide + H2O
-
 Streptococcus salivarius L-Ala + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O
-
 Streptococcus salivarius L-Arg + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Streptococcus salivarius L-Leu + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O
-
 Streptococcus salivarius L-Lys + 4-nitroaniline
-
 ?
L-Phe-4-nitroanilide + H2O
-
 Streptococcus salivarius L-Phe + 4-nitroaniline
-
 ?
Leu-4-methylcoumarin 7-amide + H2O
-
 Streptococcus salivarius Leu + 7-amino-4-methylcoumarin
-
 ?
Lys-4-methylcoumarin 7-amide + H2O
-
 Streptococcus salivarius Lys + 7-amino-4-methylcoumarin
-
 ?
tripeptides + H2O
-
 Streptococcus salivarius dipeptides + amino acids
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 substrate Lys-p-nitroanilide Streptococcus salivarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 65 low activity below and no activity above Streptococcus salivarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7 substrate Lys-p-nitroanilide Streptococcus salivarius

pH Range

pH Minimum pH Maximum Comment Organism
5.8 7.6 substrate Lys-p-nitroanilide, greater than 50% of maximum activity between pH 5.8 and pH 7.6 Streptococcus salivarius