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Literature summary for 3.4.11.18 extracted from

  • Kanudia, P.; Mittal, M.; Kumaran, S.; Chakraborti, P.
    Amino-terminal extension present in the methionine aminopeptidase type 1c of Mycobacterium tuberculosis is indispensible for its activity (2011), BMC Biochem., 12, 35.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
D117A lining the perimeter of the active site, no activity Mycobacterium tuberculosis
D131A no activity Mycobacterium tuberculosis
DELTA 2-10 30% activity with 10fold amount of enzyme in the assay relative to wild type Mycobacterium tuberculosis
DELTA 2-15 30% activity with 10fold amount of enzyme in the assay relative to wild type Mycobacterium tuberculosis
DELTA 2-20 no activity Mycobacterium tuberculosis
DELTA 2-30 no activity Mycobacterium tuberculosis
DELTA 2-40 no activity Mycobacterium tuberculosis
E219V predicted active site residue, no activity Mycobacterium tuberculosis
E238A predicted active site residue, no activity Mycobacterium tuberculosis
H114A predicted active site residue, no activity Mycobacterium tuberculosis
H193A predicted active site residue, no activity Mycobacterium tuberculosis
H212A predicted active site residue, no activity Mycobacterium tuberculosis
H88A predicted active site residue, no activity Mycobacterium tuberculosis
P19A 65% activity relative to wild type Mycobacterium tuberculosis
P19G 35% activity relative to wild type Mycobacterium tuberculosis
V18A 30% activity relative to wild type Mycobacterium tuberculosis
V18A/P19A no activity Mycobacterium tuberculosis
V18G no activity Mycobacterium tuberculosis
V18G/P19G no activity Mycobacterium tuberculosis
W236L no activity Mycobacterium tuberculosis
W255L no activity Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
amastatin
-
 Mycobacterium tuberculosis
EDTA
-
 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.36
-
 Met-Ala-Ser pH 7.5, 30°C Mycobacterium tuberculosis
2.85
-
 Met-Gly-Met-Met pH 7.5, 30°C Mycobacterium tuberculosis
3.3
-
 Met-Ala-Ser pH 7.5, 30°C Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37300
-
 x * 37300, SDS-PAGE Mycobacterium tuberculosis
39100
-
 x * 39100, SDS-PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-Ala-Ser + H2O
-
 Mycobacterium tuberculosis L-methionine + Ala-Ser
-
 ?
Met-Gly-Met-Met + H2O
-
 Mycobacterium tuberculosis L-methionine + Gly-Met-Met
-
 ?

Subunits

Subunits Comment Organism
? x * 37300, SDS-PAGE Mycobacterium tuberculosis
? x * 39100, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
methionine aminopeptidase type 1a
-
 Mycobacterium tuberculosis
methionine aminopeptidase type 1c
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 85% activity after 10 min at 50°C, Tm: 53,6°C (determination by CD spectroscopy) Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.63
-
 Met-Gly-Met-Met calculated with 29.5 kDa as molecular mass, pH 7.5, 30°C Mycobacterium tuberculosis
11.7
-
 Met-Ala-Ser calculated with 29.5 kDa as molecular mass, pH 7.5, 30°C Mycobacterium tuberculosis
1516
-
 Met-Ala-Ser calculated with 33.1 kDa as molecular mass, pH 7.5, 30°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.32
-
 Met-Ala-Ser pH 7.5, 30°C Mycobacterium tuberculosis
3.47
-
 Met-Gly-Met-Met pH 7.5, 30°C Mycobacterium tuberculosis
1156
-
 Met-Ala-Ser pH 7.5, 30°C Mycobacterium tuberculosis