Cloned (Comment) | Organism |
---|---|
genes map-1, map-2, and map-3, encoding three isozymes, DNA and amino acid sequence determination and analysis, expression as His-tagged enzymes in Escherichia coli | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Synechocystis sp. | |
H2O2 | - |
Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
Met-Ala-Ala | recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C | Synechocystis sp. | |
1.9 | - |
Met-Gly-Gly | recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C | Synechocystis sp. | |
2.1 | - |
Met-Ala-Ala | recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
2.3 | - |
Met-Gly-Gly | recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
5.2 | - |
Met-Ala-Ala | recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
5.8 | - |
Met-Gly-Gly | recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Synechocystis sp. | |
Mg2+ | activates | Synechocystis sp. | |
Mn2+ | activates | Synechocystis sp. | |
additional information | activating potencies of divalent metal ions with the three isozymes, overview | Synechocystis sp. | |
Zn2+ | activates | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
strain PCC6803, three isozymes encoded by lr0786 or map-1, slr0918 or map-2, and sll0555 or map-3 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozymes from Escherichia coli by nickel affinity chromatography and gel filtration | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-Ala-Ala + H2O | - |
Synechocystis sp. | Met + Ala-Ala | - |
? | |
Met-Ala-Met + H2O | - |
Synechocystis sp. | Met + Ala-Met | - |
? | |
Met-Ala-Ser + H2O | - |
Synechocystis sp. | Met + Ala-Ser | - |
? | |
Met-Arg-Phe + H2O | substrate of isozymes c1MetAP-Ia and c3MetAP-Ib, no activity with isozyme c2MetAP-Ia | Synechocystis sp. | Met + Arg-Phe | - |
? | |
Met-Asp-Gly + H2O | substrate of isozyme c3MetAP-Ib, no activity with the other two isozymes | Synechocystis sp. | Met + Asp-Gly | - |
? | |
Met-Gly-Gly + H2O | - |
Synechocystis sp. | Met + Gly-Gly | - |
? | |
Met-Leu-Gly + H2O | substrate of isozymes c1MetAP-Ia and c3MetAP-Ib, no activity with isozyme c2MetAP-Ia | Synechocystis sp. | Met + Leu-Gly | - |
? | |
Met-Met-Ala + H2O | substrate of isozymes c1MetAP-Ia and c3MetAP-Ib, no activity with isozyme c2MetAP-Ia | Synechocystis sp. | Met + Met-Ala | - |
? | |
additional information | substrate specificities of recombinant isozymes, overview, no activity towards tripeptides in which the N-terminal amino acid is not Met, no activity with Pro-Met and X-Gly-Gly peptides | Synechocystis sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
c1MetAP-Ia | - |
Synechocystis sp. |
c2MetAP-Ia | - |
Synechocystis sp. |
c3MetAP-Ib | - |
Synechocystis sp. |
MetAP | - |
Synechocystis sp. |
methionine aminopeptidase | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
all isozymes | Synechocystis sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | - |
Synechocystis sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.25 | - |
Met-Gly-Gly | recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
15.57 | - |
Met-Ala-Ala | recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
19.87 | - |
Met-Gly-Gly | recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
20.02 | - |
Met-Ala-Ala | recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
21.87 | - |
Met-Gly-Gly | recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C | Synechocystis sp. | |
22.07 | - |
Met-Ala-Ala | recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
isozyme c2MetAP-Ia | Synechocystis sp. |
7.5 | - |
isozyme c3MetAP-Ib | Synechocystis sp. |
8 | - |
isozyme c1MetAP-Ia | Synechocystis sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Synechocystis sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.63 | - |
Met-Gly-Gly | recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
3 | - |
Met-Ala-Ala | recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
8.63 | - |
Met-Gly-Gly | recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
9.53 | - |
Met-Ala-Ala | recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C | Synechocystis sp. | |
11.52 | - |
Met-Gly-Gly | recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C | Synechocystis sp. | |
14.72 | - |
Met-Ala-Ala | recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C | Synechocystis sp. |