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Literature summary for 3.4.11.18 extracted from

  • Atanassova, A.; Sugita, M.; Sugiura, M.; Pajpanova, T.; Ivanov, I.
    Molecular cloning, expression and characterization of three distinctive genes encoding methionine aminopeptidases in cyanobacterium Synechocystis sp. strain PCC6803 (2003), Arch. Microbiol., 180, 185-193.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes map-1, map-2, and map-3, encoding three isozymes, DNA and amino acid sequence determination and analysis, expression as His-tagged enzymes in Escherichia coli Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Synechocystis sp.
H2O2
-
Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
Met-Ala-Ala recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.
1.9
-
Met-Gly-Gly recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.
2.1
-
Met-Ala-Ala recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
2.3
-
Met-Gly-Gly recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
5.2
-
Met-Ala-Ala recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
5.8
-
Met-Gly-Gly recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Synechocystis sp.
Mg2+ activates Synechocystis sp.
Mn2+ activates Synechocystis sp.
additional information activating potencies of divalent metal ions with the three isozymes, overview Synechocystis sp.
Zn2+ activates Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
strain PCC6803, three isozymes encoded by lr0786 or map-1, slr0918 or map-2, and sll0555 or map-3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozymes from Escherichia coli by nickel affinity chromatography and gel filtration Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-Ala-Ala + H2O
-
Synechocystis sp. Met + Ala-Ala
-
?
Met-Ala-Met + H2O
-
Synechocystis sp. Met + Ala-Met
-
?
Met-Ala-Ser + H2O
-
Synechocystis sp. Met + Ala-Ser
-
?
Met-Arg-Phe + H2O substrate of isozymes c1MetAP-Ia and c3MetAP-Ib, no activity with isozyme c2MetAP-Ia Synechocystis sp. Met + Arg-Phe
-
?
Met-Asp-Gly + H2O substrate of isozyme c3MetAP-Ib, no activity with the other two isozymes Synechocystis sp. Met + Asp-Gly
-
?
Met-Gly-Gly + H2O
-
Synechocystis sp. Met + Gly-Gly
-
?
Met-Leu-Gly + H2O substrate of isozymes c1MetAP-Ia and c3MetAP-Ib, no activity with isozyme c2MetAP-Ia Synechocystis sp. Met + Leu-Gly
-
?
Met-Met-Ala + H2O substrate of isozymes c1MetAP-Ia and c3MetAP-Ib, no activity with isozyme c2MetAP-Ia Synechocystis sp. Met + Met-Ala
-
?
additional information substrate specificities of recombinant isozymes, overview, no activity towards tripeptides in which the N-terminal amino acid is not Met, no activity with Pro-Met and X-Gly-Gly peptides Synechocystis sp. ?
-
?

Synonyms

Synonyms Comment Organism
c1MetAP-Ia
-
Synechocystis sp.
c2MetAP-Ia
-
Synechocystis sp.
c3MetAP-Ib
-
Synechocystis sp.
MetAP
-
Synechocystis sp.
methionine aminopeptidase
-
Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
all isozymes Synechocystis sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50
-
Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.25
-
Met-Gly-Gly recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
15.57
-
Met-Ala-Ala recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
19.87
-
Met-Gly-Gly recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
20.02
-
Met-Ala-Ala recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
21.87
-
Met-Gly-Gly recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.
22.07
-
Met-Ala-Ala recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
isozyme c2MetAP-Ia Synechocystis sp.
7.5
-
isozyme c3MetAP-Ib Synechocystis sp.
8
-
isozyme c1MetAP-Ia Synechocystis sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
Synechocystis sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.63
-
Met-Gly-Gly recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3
-
Met-Ala-Ala recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
8.63
-
Met-Gly-Gly recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
9.53
-
Met-Ala-Ala recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
11.52
-
Met-Gly-Gly recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.
14.72
-
Met-Ala-Ala recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.