Literature summary for 3.4.11.18 extracted from

Narayanan, S.S.; Ramanujan, A.; Krishna, S.; Nampoothiri, K.M.
Purification and biochemical characterization of methionine aminopeptidase (MetAP) from Mycobacterium smegmatis mc2155 (2008), Appl. Biochem. Biotechnol., 151, 512-521.

Search for more literature for 3.4.11.18

Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethanol	complete inhibition at 10 mM	Mycolicibacterium smegmatis
actinonin	12.6% relative activity at 0.05 mM	Mycolicibacterium smegmatis
amastatin	29.7% relative activity at 0.05 mM	Mycolicibacterium smegmatis
bestatin	44.9% relative activity at 0.05 mM	Mycolicibacterium smegmatis
Cu2+	-	Mycolicibacterium smegmatis
EDTA	33.7% relative activity at 100 mM, the enzyme activity inhibited by 1 mM EDTA is restored in presence of 5 mM Mg2+	Mycolicibacterium smegmatis
Fe2+	-	Mycolicibacterium smegmatis
K+	-	Mycolicibacterium smegmatis
phenylmethylsulfonyl fluoride	29.4% relative activity at 5 mM	Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	the enzyme activity is enhanced by 1 mM Co2+	Mycolicibacterium smegmatis
Mg2+	the enzyme activity is enhanced 2fold by 1 mM Mg2+	Mycolicibacterium smegmatis
Na+	some positive effect on the enzyme activity at 5 mM	Mycolicibacterium smegmatis
NH4+	some positive effect on the enzyme activity at 5 mM	Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	the calculated molecular weight from nucleotide-derived amino acid sequence is about 32000 Da	Mycolicibacterium smegmatis
37000	-	SDS-PAGE	Mycolicibacterium smegmatis

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephadex G-100 gel filtration	Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.74	-	crude extract, at 50°C	Mycolicibacterium smegmatis
16.2	-	after 22fold purification, at 50°C	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Met-p-nitroanilide + H2O	-	Mycolicibacterium smegmatis	L-Met + p-nitroaniline	-	?
L-Met-p-nitroanilide + H2O	-	Mycolicibacterium smegmatis mc(2)155 / ATCC 700084	L-Met + p-nitroaniline	-	?

Synonyms

Synonyms	Comment	Organism
MetAP	-	Mycolicibacterium smegmatis
methionine aminopeptidase	-	Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	optimum temperature for MetAP activity against the substrate L-Met p-nitroanilide	Mycolicibacterium smegmatis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	65	the enzyme is stable at 50 °C with half-life of more than 8 h, at 50°C and 55°C, the enzyme shows stable activity over a period of 1 h with only a slight decrease of 10% in activity, at 60 °C the relative activity decreases to 50% within 50 min, whereas at 65°C the relative activity decreases to 19% and declines to 0 after a period of 50 min	Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)