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Literature summary for 3.4.11.18 extracted from

  • Hu, X.V.; Chen, X.; Han, K.C.; Mildvan, A.S.; Liu, J.O.
    Kinetic and mutational studies of the number of interacting divalent cations required by bacterial and human methionine aminopeptidases (2007), Biochemistry, 46, 12833-12843.
    View publication on PubMed
Search for more literature for 3.4.11.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Co2+ inhibitory at 0.1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 Met-Pro-p-nitroanilide in 40 mM HEPES, pH 8.0, and 100 mM NaCl, at 23°C Escherichia coli
0.576
-
 Met-Pro-p-nitroanilide in 40 mM HEPES, pH 8.0, and 100 mM NaCl, at 23°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ significant activation at 0.01 mM, at least two Co2+ ions, which may act cooperatively, are needed to promote catalysis Homo sapiens
Zn2+ slight activation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Met-peptide + H2O Escherichia coli
-
 Met + peptide
-
 ?
Met-peptide + H2O Homo sapiens
-
 Met + peptide
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens Q6UB28 mitochondrial precursor
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Superflow resin chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-peptide + H2O
-
 Escherichia coli Met + peptide
-
 ?
Met-peptide + H2O
-
 Homo sapiens Met + peptide
-
 ?
Met-Pro-p-nitroanilide + H2O
-
 Escherichia coli Pro-p-nitroanilide + Met
-
 ?
Met-Pro-p-nitroanilide + H2O
-
 Homo sapiens Pro-p-nitroanilide + Met
-
 ?

Synonyms

Synonyms Comment Organism
MetAP
-
 Escherichia coli
MetAP1D
-
 Homo sapiens
methionine aminopeptidase
-
 Escherichia coli
methionine aminopeptidase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0065
-
 Met-Pro-p-nitroanilide in 40 mM HEPES, pH 8.0, and 100 mM NaCl, at 23°C Homo sapiens
1.82
-
 Met-Pro-p-nitroanilide in 40 mM HEPES, pH 8.0, and 100 mM NaCl, at 23°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Homo sapiens