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Literature summary for 3.4.11.18 extracted from

  • Li, J.Y.; Cui, Y.M.; Chen, L.L.; Gu, M.; Li, J.; Nan, F.J.; Ye, Q.Z.
    Mutations at the S1 sites of methionine aminopeptidases from Escherichia coli and Homo sapiens reveal the residues critical for substrate specificity (2004), J. Biol. Chem., 279, 21128-21134.
    View publication on PubMed
Search for more literature for 3.4.11.18

Protein Variants

Protein Variants Comment Organism
C202A hydrolysis of Met-PSI[C(O)S]Gly-Phe similar to wild-type, relaxed specificity Homo sapiens
C59A hydrolysis of Met-Ala-Ser similar to wild-type Escherichia coli
C70A hydrolysis of Met-Ala-Ser similar to wild-type, relaxed specificity Escherichia coli
C70S relaxed specificity by opening of S1 pocket Escherichia coli
F177A reduction of kcat- and of KM-value for Met-PSI[C(O)S]Gly-Phe Escherichia coli
F197A no hydrolysis of Met-PSI[C(O)S]Gly-Phe Homo sapiens
F308A increase of kcat- and of KM-value for Met-PSI[C(O)S]Gly-Phe Homo sapiens
H211A reduction of kcat- and of KM-value for Met-PSI[C(O)S]Gly-Phe Homo sapiens
H79A reduction of kcat- and of KM-value for Met-PSI[C(O)S]Gly-Phe Escherichia coli
P191A hydrolysis of Met-Ala-Ser similar to wild-type Homo sapiens
W221A no hydrolysis of Met-PSI[C(O)S]Gly-Phe Escherichia coli
W352A increase of kcat- and of KM-value for Met-PSI[C(O)S]Gly-Phe Homo sapiens
Y194A no hydrolysis of Met-PSI[C(O)S]Gly-Phe Homo sapiens
Y62A hydrolysis of Met-Ala-Ser similar to wild-type Escherichia coli
Y65A no hydrolysis of Met-PSI[C(O)S]Gly-Phe Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 L-Leu-PSI[C(O)S]Gly-L-Phe mutant C70S, pH 7.0, 22°C Escherichia coli
0.24
-
 L-Phe-PSI[C(O)S]Gly-L-Phe mutant C70S, pH 7.0, 22°C Escherichia coli
0.35
-
 L-Leu-PSI[C(O)S]Gly-L-Phe mutant C70A, pH 7.0, 22°C Escherichia coli
0.35
-
 L-Met-PSI[C(O)S]Gly-L-Phe wild-type, pH 7.0, 22°C Escherichia coli
0.68
-
 L-Met-PSI[C(O)S]Gly-L-Phe mutant C70S, pH 7.0, 22°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AE18 type I isoform
-
Homo sapiens
-
 type I isoform
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-PSI[C(O)S]Gly-L-Phe + H2O
-
 Escherichia coli ?
-
 ?
L-Met-L-Ala-L-Ser + H2O
-
 Homo sapiens L-Met + L-Ala-L-Ser
-
 ?
L-Met-L-Ala-L-Ser + H2O
-
 Escherichia coli L-Met + L-Ala-L-Ser
-
 ?
L-Met-PSI[C(O)S]Gly-L-Phe + H2O
-
 Homo sapiens ?
-
 ?
L-Met-PSI[C(O)S]Gly-L-Phe + H2O
-
 Escherichia coli ?
-
 ?
L-Phe-PSI[C(O)S]Gly-L-Phe + H2O
-
 Homo sapiens ?
-
 ?
L-Phe-PSI[C(O)S]Gly-L-Phe + H2O
-
 Escherichia coli ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3
-
 L-Phe-PSI[C(O)S]Gly-L-Phe mutant C70S, pH 7.0, 22°C Escherichia coli
4.04
-
 L-Leu-PSI[C(O)S]Gly-L-Phe mutant C70S, pH 7.0, 22°C Escherichia coli
4.55
-
 L-Phe-PSI[C(O)S]Gly-L-Phe mutant C70S, pH 7.0, 22°C Escherichia coli
20.8
-
 L-Leu-PSI[C(O)S]Gly-L-Phe mutant C70A, pH 7.0, 22°C Escherichia coli
22.5
-
 L-Met-PSI[C(O)S]Gly-L-Phe wild-type, pH 7.0, 22°C Escherichia coli
30.8
-
 L-Met-PSI[C(O)S]Gly-L-Phe mutant C70S, pH 7.0, 22°C Escherichia coli