Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.1 extracted from

  • Hanson, H.; Frohne, M.
    Crystalline leucine aminopeptidase from lens (1976), Methods Enzymol., 45, 504-521.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Bos taurus

General Stability

General Stability Organism
lyophilization, inactivation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Chloromethylketones
-
Bos taurus
Zn2+
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
L-Leu-phenylazophenylamide
-
Bos taurus
0.25
-
L-Leu-2-naphthylamide
-
Bos taurus
1.1
-
L-Leu-4-nitroanilide
-
Bos taurus
7.2
-
Leu-Gly similar value Bos taurus
17
-
leucinamide similar value Bos taurus
17.5
-
L-Leu-hydrazide
-
Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation Bos taurus
Mn2+ activation Bos taurus
Zn2+ 6-12 gatom per mol Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
326000 360000
-
Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
lens
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
assay methods Saccharomyces cerevisiae
additional information
-
assay methods Bos taurus

Storage Stability

Storage Stability Organism
4°C, 0.1 M Tris-HCl buffer, 1 year Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amino acid amides + H2O fast hydrolysis: L-Leu Bos taurus amino acid + NH3
-
?
L-amino acid-peptide + H2O free N-terminal alpha-amino group required Bos taurus amino acid + peptide
-
?
L-Leu-2-naphthylamide + H2O
-
Bos taurus L-Leu + 2-naphthylamine
-
?
L-Leu-hydrazide
-
Bos taurus ?
-
?
L-Leu-phenylazophenylamide
-
Bos taurus ?
-
?
L-leucine amide + H2O
-
Bos taurus L-leucine + NH3
-
?
Leu-Gly + H2O
-
Bos taurus Leu + Gly
-
?
additional information esterase activity Bos taurus ?
-
?
additional information transferase activity Bos taurus ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
soluble enzyme: 5 min, immobilized enzyme: 10 min Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.4 esterase activity Bos taurus