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Literature summary for 3.4.11.1 extracted from
- Immunoaffininty purification and characterization of leucine aminopeptidase from human liver (1986), J. Biol. Chem., 61, 10744-10748.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Homo sapiens |  |
| amastatin | - |
Homo sapiens | |
| bestatin | - |
Homo sapiens | |
| EDTA | reactivation by divalent cations | Homo sapiens | |
| iodoacetic acid | - |
Homo sapiens | |
| N-ethylmaleimide | - |
Homo sapiens | |
| p-chloromercuribenzoate | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.02 | - |
leucinamide | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Homo sapiens | |
| Mn2+ | activation | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 326000 | 360000 | non-denaturing PAGE, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 58 | - |
substrate L-Leu-amide | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 100 mM Tris-HCl buffer, pH 8.0, 6 weeks | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amino acid amides + H2O | - |
Homo sapiens | amino acid + NH3 | - |
? | |
| L-Leu-p-nitroanilide + H2O | no hydrolysis | Homo sapiens | L-leucine + p-nitroaniline | - |
? | |
| L-leucine amide + H2O | - |
Homo sapiens | L-leucine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 3 * ab, a: 53000, b: 65000, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
2 weeks | Homo sapiens |
| 37 | - |
1 day | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 10.4 | 10.7 | - |
Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Homo sapiens |
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