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Literature summary for 3.4.11.1 extracted from

  • Jarocki, V.M.; Santos, J.; Tacchi, J.L.; Raymond, B.B.; Deutscher, A.T.; Jenkins, C.; Padula, M.P.; Djordjevic, S.P.
    MHJ_0461 is a multifunctional leucine aminopeptidase on the surface of Mycoplasma hyopneumoniae (2015), Open biology, 5, 140175.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Mycoplasma hyopneumoniae

Inhibitors

Inhibitors Comment Organism Structure
amastatin
-
 Mycoplasma hyopneumoniae
bestatin
-
 Mycoplasma hyopneumoniae
EDTA
-
 Mycoplasma hyopneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ inhibits activity Mycoplasma hyopneumoniae
Co2+ enhances activity Mycoplasma hyopneumoniae
Cu2+ inhibits activity Mycoplasma hyopneumoniae
Mg2+ enhances activity Mycoplasma hyopneumoniae
Mn2+ enhances activity Mycoplasma hyopneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51400
-
 calculated from cDNA Mycoplasma hyopneumoniae
500000
-
 native PAGE, rMHJ_0461 forms multimers between 500 kDa-800 kDa Mycoplasma hyopneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycoplasma hyopneumoniae MHJ_0461 binds heparin and interacts with DNA ?
-
 ?
additional information Mycoplasma hyopneumoniae rMHJ_0461 binds plasminogen and facilitates plasmin conversion ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycoplasma hyopneumoniae Q4A9M4
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Mycoplasma hyopneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-7-amido-4-methylcoumarin + H2O enzyme shows only weak activity with this substrate Mycoplasma hyopneumoniae glycine + 7-amino-4-methylcoumarin
-
 ?
L-Ala-7-amido-4-methylcoumarin + H2O
-
 Mycoplasma hyopneumoniae L-Ala + 7-amino-4-methylcoumarin
-
 ?
L-Arg-7-amido-4-methylcoumarin + H2O
-
 Mycoplasma hyopneumoniae L-Arg + 7-amino-4-methylcoumarin
-
 ?
L-Ile-7-amido-4-methylcoumarin + H2O enzyme shows only weak activity with this substrate Mycoplasma hyopneumoniae L-isoleucine + 7-amino-4-methylcoumarin
-
 ?
L-Leu-7-amido-4-methylcoumarin + H2O
-
 Mycoplasma hyopneumoniae L-Leu + 7-amino-4-methylcoumarin
-
 ?
L-Met-7-amido-4-methylcoumarin + H2O
-
 Mycoplasma hyopneumoniae L-Met + 7-amino-4-methylcoumarin
-
 ?
L-Phe-7-amido-4-methylcoumarin + H2O
-
 Mycoplasma hyopneumoniae L-Phe + 7-amino-4-methylcoumarin
-
 ?
L-Val-7-amido-4-methylcoumarin + H2O enzyme shows only weak activity with this substrate Mycoplasma hyopneumoniae L-Val + 7-amino-4-methylcoumarin
-
 ?
additional information MHJ_0461 binds heparin and interacts with DNA Mycoplasma hyopneumoniae ?
-
 ?
additional information rMHJ_0461 binds plasminogen and facilitates plasmin conversion Mycoplasma hyopneumoniae ?
-
 ?

Subunits

Subunits Comment Organism
homohexamer
-
 Mycoplasma hyopneumoniae

Synonyms

Synonyms Comment Organism
rMHJ_0461
-
 Mycoplasma hyopneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mycoplasma hyopneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Mycoplasma hyopneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
7.2 10
-
 Mycoplasma hyopneumoniae

General Information

General Information Comment Organism
physiological function rMHJ_0461 binds plasminogen and facilitates plasmin conversion Mycoplasma hyopneumoniae