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Literature summary for 3.4.11.1 extracted from

  • Hernandez-Moreno, A.; Perdomo-Abundez, F.; Perez-Medina Martinez, V.; Luna-Barcenas, G.; Villasenor-Ortega, F.; Perez, N.; Lopez-Morales, C.; Flores-Ortiz, L.; Medina-Rivero, E.
    Structural and functional characterization of a recombinant leucine aminopeptidase (2015), J. Mol. Catal. B, 113, 39-46.
No PubMed abstract available
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein in Escherichia coli Vibrio proteolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Vibrio proteolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31880
-
 mass spectroscopy Vibrio proteolyticus

Organism

Organism UniProt Comment Textmining
Vibrio proteolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-4-nitroanilide + H2O
-
 Vibrio proteolyticus L-Leu + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
rLAP
-
 Vibrio proteolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Vibrio proteolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
54
-
 folded rLAP Tm: 54°C Vibrio proteolyticus
54
-
 unfolded rLAP Tm: 54°C Vibrio proteolyticus
85
-
 folded rLAP Tm: 54°C Vibrio proteolyticus
88
-
 folded rLAP Tm: 54°C Vibrio proteolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 when pH decreases down to 6.0 using phosphate buffer, the specific activity of rLAP augments to 31 U/mg after 72 h incubation Vibrio proteolyticus
8
-
 assay tricine buffer: activity remains below 2.5 U/mg Vibrio proteolyticus

pH Range

pH Minimum pH Maximum Comment Organism
4 6 assay citrate buffer: activity remains below 2.5 U/mg Vibrio proteolyticus
7 8 an increase to 15 U/mg is observed after 72 h of incubation in phosphate buffer at pH 7.0 and 8.0 Vibrio proteolyticus
8.5 9 a subtle increase in activity is observed using carbonate buffer at pH 8.5 and 9.0 Vibrio proteolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
 when the pH increased to 10.0 the activity is totally lost after 72 h at 37°C Vibrio proteolyticus