Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
D347N | mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity | Solanum lycopersicum |
D347R | inactive mutant with disrupted hexameric structure has increased chaperone activity | Solanum lycopersicum |
E429R | inactive mutant with disrupted hexameric structure has increased chaperone activity | Solanum lycopersicum |
E429V | mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity | Solanum lycopersicum |
K354E | inactive mutant with disrupted hexameric structure has increased chaperone activity | Solanum lycopersicum |
K354R | mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity | Solanum lycopersicum |
additional information | LAP-A exhibits chaperone activity which is independant of its peptidase activity | Solanum lycopersicum |
R431A | mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Solanum lycopersicum | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | Q10712 | - |
- |
Solanum lycopersicum | Q8GZD8 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Leu + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | disruption of hexameric structure increases in vitro chaperone activity | Solanum lycopersicum |
Synonyms | Comment | Organism |
---|---|---|
LAP-A | stress-induced acidic LAPs, LAP-A exhibits chaperone activity | Solanum lycopersicum |
LAP-N | neutral LAP, LAP-N exhibtis chaperone activity | Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
malfunction | disruption of the LAP-A hexameric structure increases chaperone activity | Solanum lycopersicum |
physiological function | LAP-A exhibits chaperone activity which is independant of its peptidase activity | Solanum lycopersicum |