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Literature summary for 3.4.11.1 extracted from

  • Dura, M.A.; Receveur-Brechot, V.; Andrieu, J.P.; Ebel, C.; Schoehn, G.; Roussel, A.; Franzetti, B.
    Characterization of a TET-like aminopeptidase complex from the hyperthermophilic archaeon Pyrococcus horikoshii (2005), Biochemistry, 44, 3477-3486.
    View publication on PubMed
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

Inhibitors

Inhibitors Comment Organism Structure
bestatin 0.05 mM 35.6% inhibition Pyrococcus horikoshii
EDTA 2 mM, 80% inhibition Pyrococcus horikoshii
Mn2+ 0.5 mM, around 50% inhibition Pyrococcus horikoshii
additional information insensitive to chamostatin, E-64, leupeptin, pepstatin, aprotinin and puromycin Pyrococcus horikoshii
phosphoramidon 1 mM, 25% inhibition Pyrococcus horikoshii
Zn2+ 0.5 mM, around 50% inhibition Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ optimally stimulates at 0.5-10 mM Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
475000
-
 gel filtration Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O59196
-
-
Pyrococcus horikoshii OT-3 O59196
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine 4-nitroanilide + H2O 10.8% of the activity compared to Leu-4-nitroanilide Pyrococcus horikoshii L-alanine + 4-nitroaniline
-
 ?
L-alanine 4-nitroanilide + H2O 10.8% of the activity compared to Leu-4-nitroanilide Pyrococcus horikoshii OT-3 L-alanine + 4-nitroaniline
-
 ?
L-isoleucine 4-nitroanilide + H2O 14.3% of the activity compared to Leu-4-nitroanilide Pyrococcus horikoshii L-isoleucine + 4-nitroaniline
-
 ?
L-isoleucine 4-nitroanilide + H2O 14.3% of the activity compared to Leu-4-nitroanilide Pyrococcus horikoshii OT-3 L-isoleucine + 4-nitroaniline
-
 ?
L-leucine 4-nitroanilide + H2O
-
 Pyrococcus horikoshii L-leucine + 4-nitroaniline
-
 ?
L-leucine 4-nitroanilide + H2O
-
 Pyrococcus horikoshii OT-3 L-leucine + 4-nitroaniline
-
 ?
L-methionine 4-nitroanilide + H2O 26.3% of the activity compared to Leu-4-nitroanilide Pyrococcus horikoshii L-methionine + 4-nitroaniline
-
 ?
L-methionine 4-nitroanilide + H2O 26.3% of the activity compared to Leu-4-nitroanilide Pyrococcus horikoshii OT-3 L-methionine + 4-nitroaniline
-
 ?
additional information when assayed with short chromogenic peptides, PhTET2 is an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity Pyrococcus horikoshii ?
-
 ?
additional information when assayed with short chromogenic peptides, PhTET2 is an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity Pyrococcus horikoshii OT-3 ?
-
 ?

Subunits

Subunits Comment Organism
dodecamer
-
 Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
PH1527
-
 Pyrococcus horikoshii
PhTET2
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
 Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life: 10.03 h Pyrococcus horikoshii
90
-
 half-life: 3.0 h Pyrococcus horikoshii
95
-
 half-life: 59.4 min Pyrococcus horikoshii
100
-
 half-life: 24.8 min Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 PIPES buffer Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 7.0: about 60% of maximal activity, pH 9.0: about 65% of maximal activity Pyrococcus horikoshii