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Literature summary for 3.4.11.1 extracted from

  • Jia, H.; Terkawi, M.A.; Aboge, G.O.; Goo, Y.K.; Luo, Y.; Li, Y.; Yamagishi, J.; Nishikawa, Y.; Igarashi, I.; Sugimoto, C.; Fujisaki, K.; Xuan, X.
    Characterization of a leucine aminopeptidase of Babesia gibsoni (2009), Parasitology, 136, 945-952.
    View publication on PubMed
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of GST-tagged LAP in Escherichia coli DH12S cells Babesia gibsoni

Inhibitors

Inhibitors Comment Organism Structure
bestatin
-
 Babesia gibsoni
Ca2+
-
 Babesia gibsoni
Cu2+
-
 Babesia gibsoni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 L-proline-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
0.095
-
 L-alanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
0.102
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
0.108
-
 L-phenylalanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
0.109
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.0, 37°C, native enzyme Babesia gibsoni
0.189
-
 L-arginine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates moderately Babesia gibsoni
Mg2+ activates slightly Babesia gibsoni
Mn2+ activates, best divalent cation Babesia gibsoni
additional information the enzyme is strongly dependent on metal divalent cations Babesia gibsoni
Ni2+ activates slightly Babesia gibsoni
Zn2+ activates slightly Babesia gibsoni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57000, native enzyme, SDS-PAGE, x * 85000, recombinant GST-tagged enzyme, SDS-PAGE Babesia gibsoni
85000
-
 x * 57000, native enzyme, SDS-PAGE, x * 85000, recombinant GST-tagged enzyme, SDS-PAGE Babesia gibsoni

Organism

Organism UniProt Comment Textmining
Babesia gibsoni C5NSV0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
merozoite
-
 Babesia gibsoni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine-4-methylcoumaryl-7-amide + H2O
-
 Babesia gibsoni L-alanine + 7-amino-4-methylcoumarine
-
 ?
L-arginine-4-methylcoumaryl-7-amide + H2O
-
 Babesia gibsoni L-arginine + 7-amino-4-methylcoumarine
-
 ?
L-leucine-4-methylcoumaryl-7-amide + H2O
-
 Babesia gibsoni L-leucine + 7-amino-4-methylcoumarine
-
 ?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
-
 Babesia gibsoni L-phenylalanine + 7-amino-4-methylcoumarine
-
 ?
L-proline-4-methylcoumaryl-7-amide + H2O
-
 Babesia gibsoni L-proline + 7-amino-4-methylcoumarine
-
 ?
additional information substrate specificity, overview Babesia gibsoni ?
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, native enzyme, SDS-PAGE, x * 85000, recombinant GST-tagged enzyme, SDS-PAGE Babesia gibsoni

Synonyms

Synonyms Comment Organism
LAP
-
 Babesia gibsoni
leucine aminopeptidase
-
 Babesia gibsoni
M17 leucyl aminopeptidase
-
 Babesia gibsoni
More the enzyme belongs to the M17 family leucine aminopeptidases Babesia gibsoni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Babesia gibsoni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.03
-
 L-proline-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
8.2
-
 L-alanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
24.61
-
 L-phenylalanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
37.79
-
 L-arginine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
198.8
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Babesia gibsoni

pH Range

pH Minimum pH Maximum Comment Organism
7 10 activity range Babesia gibsoni

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.011
-
 L-proline-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
0.086
-
 L-alanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
0.227
-
 L-phenylalanine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
0.752
-
 L-arginine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni
1.942
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.0, 37°C, recombinant GST-tagged enzyme Babesia gibsoni