Cloned (Comment) | Organism |
---|---|
phylogenetic tree, expression of GST-tagged LAP in Escherichia coli strain BL21, construction of a HA-tagged enzyme | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | - |
Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-phenylalanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.107 | - |
L-leucine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.124 | - |
L-proline-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.169 | - |
L-alanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.426 | - |
L-arginine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Toxoplasma gondii | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Toxoplasma gondii | |
Mn2+ | activates, best metal ion | Toxoplasma gondii | |
additional information | the enzyme activity strongly depends on metal ions, only slight activition by Fe2+, Mg2+, Ca2+, Cu2+, and Zn2+ | Toxoplasma gondii | |
Ni2+ | activates | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 85000, recombinant GST-tagged enzyme, SDS-PAGE, x * 60000, native enzyme, SDS-PAGE | Toxoplasma gondii |
85000 | - |
x * 85000, recombinant GST-tagged enzyme, SDS-PAGE, x * 60000, native enzyme, SDS-PAGE | Toxoplasma gondii |
500000 | - |
recombinant enzyme, native PAGE | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Toxoplasma gondii RH | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged LAP from Escherichia coli strain BL21 by glutathione affinity chromatography | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii | L-alanine + 7-amino-4-methylcoumarin | - |
? | |
L-alanine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii RH | L-alanine + 7-amino-4-methylcoumarin | - |
? | |
L-arginine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii | L-arginine + 7-amino-4-methylcoumarin | - |
? | |
L-arginine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii RH | L-arginine + 7-amino-4-methylcoumarin | - |
? | |
L-leucine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii | L-leucine + 7-amino-4-methylcoumarin | - |
? | |
L-leucine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii RH | L-leucine + 7-amino-4-methylcoumarin | - |
? | |
L-phenylalanine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii | L-phenylalanine + 7-amino-4-methylcoumarin | - |
? | |
L-phenylalanine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii RH | L-phenylalanine + 7-amino-4-methylcoumarin | - |
? | |
L-proline-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii | L-proline + 7-amino-4-methylcoumarin | - |
? | |
L-proline-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii RH | L-proline + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 85000, recombinant GST-tagged enzyme, SDS-PAGE, x * 60000, native enzyme, SDS-PAGE | Toxoplasma gondii |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Toxoplasma gondii |
leucine aminopeptidase | - |
Toxoplasma gondii |
More | the enzyme belongs to the M17 family leucine aminopeptidases | Toxoplasma gondii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Toxoplasma gondii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
L-phenylalanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.0016 | - |
L-proline-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.03 | - |
L-alanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.041 | - |
L-arginine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.089 | - |
L-leucine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Toxoplasma gondii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
L-phenylalanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.013 | - |
L-proline-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.095 | - |
L-arginine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.18 | - |
L-alanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
0.832 | - |
L-leucine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii |