Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide | pH 8.0, 31°C | Sus scrofa | |
3 | - |
L-leucine-4-nitroanilide | pH 8.0, 31°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sus scrofa | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.044 | - |
kidney enzyme, substrate is N-(6-methoxy-2-methylpyridin-3-yl)-(S)-2-amino-4-methylpentanamide | Sus scrofa |
5.1 | - |
kidney enzyme, substrate is L-leucine-4-nitroanilide | Sus scrofa |
6.5 | - |
kidney enzyme, substrate is N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide | Sus scrofa |
8.4 | - |
kidney enzyme, substrate is (S)-2-amino-N-(6-(dimethylamino)pyridin-3-yl)-4-methylpentanamide | Sus scrofa |
9.6 | - |
kidney enzyme, substrate is L-leucyl-4-methylcoumaryl 7-amide | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-amino-N-(6-(dimethylamino)pyridin-3-yl)-4-methylpentanamide + H2O | - |
Sus scrofa | ? | - |
? | |
L-leucine-4-methylcoumaryl-7-amide + H2O | - |
Sus scrofa | L-leucine + 7-amino-4-methylcoumarine | - |
? | |
L-leucine-4-nitroanilide + H2O | - |
Sus scrofa | L-leucine + 4-nitroaniline | - |
? | |
additional information | substrate synthesis and assay method optimization, overview | Sus scrofa | ? | - |
? | |
N-(6-methoxy-2-methylpyridin-3-yl)-(S)-2-amino-4-methylpentanamide + H2O | - |
Sus scrofa | ? | - |
? | |
N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide + H2O | - |
Sus scrofa | ? | - |
? | |
N-(tert-butoxycarbonyl)-L-leucine + H2O | - |
Sus scrofa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Sus scrofa |
leucine aminopeptidase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
31 | - |
- |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sus scrofa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.007 | - |
pH 8.0, 31°C, versus substrate L-leucine-4-nitroanilide | Sus scrofa | bestatin | |
0.025 | - |
pH 8.0, 31°C, versus substrate N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide | Sus scrofa | bestatin |