Application | Comment | Organism |
---|---|---|
medicine | antimalarial activity of aminopeptidase inhibitors | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expression in Sf9 cells | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | antimalarial activity of aminopeptidase inhibitors | Plasmodium falciparum | |
bestatin methyl ester | - |
Plasmodium falciparum | |
Cu2+ | 1.0 mM | Plasmodium falciparum | |
EDTA | 10 mM inhibition | Plasmodium falciparum | |
nitrobestatin | - |
Plasmodium falciparum | |
o-phenanthroline | 10 mM, abolishes enzyme activity in presence or absence of Co2+ | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00792 | - |
Phe-4-methylcoumaryl-7-amide | - |
Plasmodium falciparum | |
0.01212 | - |
Leu-4-methylcoumaryl-7-amide | - |
Plasmodium falciparum | |
0.0529 | - |
Ala-4-methylcoumaryl-7-amide | - |
Plasmodium falciparum | |
0.2349 | - |
Pro-4-methylcoumaryl-7-amide | - |
Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | addition of the ion prior to mixing with substrate increases activity up to 24fold | Plasmodium falciparum | |
Mg2+ | addition of the ion prior to mixing with substrate increases activity up to 8fold | Plasmodium falciparum | |
Mn2+ | addition of the ion prior to mixing with substrate increases activity up to 24fold | Plasmodium falciparum | |
Ni2+ | addition of the ion prior to mixing with substrate increases activity up to 10fold | Plasmodium falciparum | |
Zn2+ | addition of the ion prior to mixing with substrate increases activity up to 4fold | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
6 * 60000, SDS-PAGE | Plasmodium falciparum |
320000 | - |
gel filtration | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Plasmodium falciparum | the enzyme generates and regulates the internal pool of free amino acids and therefore represents a target for antimalarial drugs | ? | - |
? | |
additional information | Plasmodium falciparum M17 | the enzyme generates and regulates the internal pool of free amino acids and therefore represents a target for antimalarial drugs | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8IL11 | - |
- |
Plasmodium falciparum M17 | Q8IL11 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | PfLAP is produced by all stages in the intra-erythrocytic developmental cycle of malaria but is most highly expressed by trophozoites | Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-4-methylcoumaryl-7-amide + H2O | - |
Plasmodium falciparum | Ala + 7-amino-4-methylcoumarin | - |
? | |
Ala-4-methylcoumaryl-7-amide + H2O | - |
Plasmodium falciparum M17 | Ala + 7-amino-4-methylcoumarin | - |
? | |
Leu-4-methylcoumaryl-7-amide + H2O | - |
Plasmodium falciparum | Leu + 7-amino-4-methylcoumarin | - |
? | |
Leu-4-methylcoumaryl-7-amide + H2O | - |
Plasmodium falciparum M17 | Leu + 7-amino-4-methylcoumarin | - |
? | |
additional information | the enzyme generates and regulates the internal pool of free amino acids and therefore represents a target for antimalarial drugs | Plasmodium falciparum | ? | - |
? | |
additional information | the enzyme generates and regulates the internal pool of free amino acids and therefore represents a target for antimalarial drugs | Plasmodium falciparum M17 | ? | - |
? | |
Phe-4-methylcoumaryl-7-amide + H2O | - |
Plasmodium falciparum | Phe + 7-amino-4-methylcoumarin | - |
? | |
Phe-4-methylcoumaryl-7-amide + H2O | - |
Plasmodium falciparum M17 | Phe + 7-amino-4-methylcoumarin | - |
? | |
Pro-4-methylcoumaryl-7-amide + H2O | - |
Plasmodium falciparum | Pro + 7-amino-4-methylcoumarin | - |
? | |
Pro-4-methylcoumaryl-7-amide + H2O | - |
Plasmodium falciparum M17 | Pro + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 60000, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
PfLAP | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
Ala-4-methylcoumaryl-7-amide | - |
Plasmodium falciparum | |
0.00059 | - |
Pro-4-methylcoumaryl-7-amide | - |
Plasmodium falciparum | |
0.00145 | - |
Phe-4-methylcoumaryl-7-amide | - |
Plasmodium falciparum | |
0.039 | - |
Leu-4-methylcoumaryl-7-amide | - |
Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000027 | - |
nitrobestatin | inhibitory activity against recombinant enzyme | Plasmodium falciparum | |
0.000025 | - |
bestatin | inhibitory activity against recombinant enzyme | Plasmodium falciparum | |
0.000138 | - |
bestatin methyl ester | inhibitory activity against recombinant enzyme | Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.008 | - |
inhibitory activity against recombinant enzyme | Plasmodium falciparum | nitrobestatin | |
0.0149 | - |
inhibitory activity against recombinant enzyme | Plasmodium falciparum | bestatin | |
0.0205 | - |
inhibitory activity against recombinant enzyme | Plasmodium falciparum | bestatin methyl ester |