Literature summary for 3.4.11.1 extracted from

Herrera-Camacho, I.; Rosas-Murrieta, N.H.; Rojo-Dominguez, A.; Millan, L.; Reyes-Leyva, J.; Santos-Lopez, G.; Suarez-Rendueles, P.
Biochemical characterization and structural prediction of a novel cytosolic leucyl aminopeptidase of the M17 family from Schizosaccharomyces pombe (2007), FEBS J., 274, 6228-6240.

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Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	1 mM, 38% inhibition	Schizosaccharomyces pombe
Ba2+	-	Schizosaccharomyces pombe
bestatin	100 ng/ml, 29% inhibition, competitive	Schizosaccharomyces pombe
Ca2+	-	Schizosaccharomyces pombe
Chloroquine	1 mM, 70% inhibition	Schizosaccharomyces pombe
Cu2+	-	Schizosaccharomyces pombe
EDTA	1 mM, 56% inhibition	Schizosaccharomyces pombe
leupeptin	0.01 mg/ml, 12% inhibition	Schizosaccharomyces pombe
Zn2+	-	Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.6	-	Lys-4-nitroanilide	pH 8.5, 37°C	Schizosaccharomyces pombe
3.7	-	Arg-4-nitroanilide	pH 8.5, 37°C	Schizosaccharomyces pombe
4.4	-	Met-4-nitroanilide	pH 8.5, 37°C	Schizosaccharomyces pombe
4.5	-	Leu-4-nitroanilide	pH 8.5, 37°C	Schizosaccharomyces pombe

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Schizosaccharomyces pombe	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	enhances activity	Schizosaccharomyces pombe
Mn2+	maximal (5fold) activation at 0.5 mM or higher	Schizosaccharomyces pombe
Ni2+	enhances activity	Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	6 * 54000, SDS-PAGE	Schizosaccharomyces pombe
320000	-	gel filtration	Schizosaccharomyces pombe

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	Q09735	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
196.9	-	-	Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ala-4-nitroanilide + H2O	-	Schizosaccharomyces pombe	Ala + 4-nitroaniline	-	?
Arg-4-nitroanilide + H2O	-	Schizosaccharomyces pombe	Arg + 4-nitroaniline	-	?
Leu-4-nitroanilide + H2O	-	Schizosaccharomyces pombe	Leu + 4-nitroaniline	-	?
Lys-4-nitroanilide + H2O	-	Schizosaccharomyces pombe	Lys + 4-nitroaniline	-	?
Met-4-nitroanilide + H2O	-	Schizosaccharomyces pombe	Met + 4-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 54000, SDS-PAGE	Schizosaccharomyces pombe

Synonyms

Synonyms	Comment	Organism
LAP yspII	-	Schizosaccharomyces pombe
leucyl aminopeptidase yspII	-	Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Schizosaccharomyces pombe

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00014	-	bestatin	-	Schizosaccharomyces pombe

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Release 2023.1 (January 2023)