Cloned (Comment) | Organism |
---|---|
isozyme ERAP1, DNA and amino acid sequence determination and analysis, expression analysis in EBV-B and tumor cell lines, recombinant expression in HeLa cells | Homo sapiens |
isozyme ERAP2, DNA and amino acid sequence determination and analysis, expression analysis in EBV-B and tumor cell lines, recombinant expression in HeLa cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
x * 115000, ERAP1, SDS-PAGE | Homo sapiens |
115000 | - |
x * 115000, ERAP2, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme is involved in peptide trimming, e.g. for the generation of most HLA class I-binding peptide, in the endoplasmic reticulum in concert with other aminopeptidases, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme ERAP1 | - |
Homo sapiens | - |
isozyme ERAP2 | - |
Homo sapiens | Q6P179 | two isozyme ERAP2 | - |
Purification (Comment) | Organism |
---|---|
by protein G affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
1102 cell | melanoma cell line | Homo sapiens | - |
1182 cell | melanoma cell line | Homo sapiens | - |
1290-2 cell | melanoma cell line | Homo sapiens | - |
1704 cell | melanoma cell line | Homo sapiens | - |
501 cell | melanoma cell line | Homo sapiens | - |
A-431 cell | - |
Homo sapiens | - |
A-549 cell | - |
Homo sapiens | - |
B-lymphoblastoid cell line | 15 EBV-B lymphoblastoid cell lines, overview | Homo sapiens | - |
BT20 cell | - |
Homo sapiens | - |
Calu1 cell | - |
Homo sapiens | - |
Colo38 cell | melanoma cell line | Homo sapiens | - |
DAUDI cell | - |
Homo sapiens | - |
EBV-B cell | 15 lymphoblastoid cell lines, overview | Homo sapiens | - |
FO-1 cell | melanoma cell line | Homo sapiens | - |
H494 cell | - |
Homo sapiens | - |
HO-1 cell | melanoma cell line | Homo sapiens | - |
HT-29 cell | - |
Homo sapiens | - |
IR-8 cell | melanoma cell line | Homo sapiens | - |
JAR cell | - |
Homo sapiens | - |
JEG-3 cell | - |
Homo sapiens | - |
K-562 cell | - |
Homo sapiens | - |
KJ29 cell | - |
Homo sapiens | - |
LoVo cell | - |
Homo sapiens | - |
LS174T cell | - |
Homo sapiens | - |
M10 cell | melanoma cell line | Homo sapiens | - |
M14 cell | melanoma cell line | Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
MEL249 cell | melanoma cell line | Homo sapiens | - |
MOLT-4 cell | - |
Homo sapiens | - |
additional information | quantitative analysis of isozyme expression in EBV-B and diverse cancer cell lines, overview | Homo sapiens | - |
MRN-1 cell | melanoma cell line | Homo sapiens | - |
SK-BR3 cell | - |
Homo sapiens | - |
SK-MEL37 cell | melanoma cell line | Homo sapiens | - |
SK-MEL93 cell | melanoma cell line | Homo sapiens | - |
SP6.5 cell | - |
Homo sapiens | - |
STP-1 cell | melanoma cell line | Homo sapiens | - |
T24 cell | - |
Homo sapiens | - |
U-937 cell | - |
Homo sapiens | - |
WI-L2 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginyl-7-amido-4-methylcoumarin + H2O | ERAP2 preferentially hydrolyzes Arg-aminomethylcoumarin and Lys-aminomethylcoumarin | Homo sapiens | L-arginine + 7-amino-4-methylcoumarin | - |
? | |
L-leucyl-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | L-leucine + 7-amino-4-methylcoumarin | - |
? | |
L-leucyl-7-amido-4-methylcoumarin + H2O | ERAP1 preferentially hydrolyzes Leu-aminoacyl-aminomethylcoumarin | Homo sapiens | L-leucine + 7-amino-4-methylcoumarin | - |
? | |
L-lysyl-7-amido-4-methylcoumarin + H2O | ERAP2 preferentially hydrolyzes Arg-aminomethylcoumarin and Lys-aminomethylcoumarin | Homo sapiens | L-lysine + 7-amino-4-methylcoumarin | - |
? | |
leucyl-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | leucine + 7-amino-4-methylcoumarin | - |
? | |
additional information | the enzyme is involved in peptide trimming, e.g. for the generation of most HLA class I-binding peptide, in the endoplasmic reticulum in concert with other aminopeptidases, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 115000, ERAP1, SDS-PAGE | Homo sapiens |
? | x * 115000, ERAP2, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
endoplasmic reticulum aminopeptidase | - |
Homo sapiens |
ERAP | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |