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Literature summary for 3.4.11.1 extracted from

  • Straeter, N.; Lipscomb, W.N.
    Leucine aminopeptidase (2004), Handbook of Metalloproteins (Messerschmidt, A. ; Huber R. Wieghardt, K. ; Poulos, T. , eds. ) Wiley, 3, 199-207.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
amastatin natural inhibitor Sus scrofa
Amino acid hydroxamates
-
Sus scrofa
bestatin natural inhibitor Sus scrofa
L-leucylthiol
-
Sus scrofa
leucinal
-
Sus scrofa
leucine phosphonic acid
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Sus scrofa
0.06
-
Leu-Phe Zn2+-Zn2+-enzyme form Sus scrofa
1
-
Gly-Phe Zn2+-Zn2+-enzyme form Sus scrofa
2
-
Leu-4-nitroanilide Mg2+-Zn2+-enzyme form Sus scrofa
4.1
-
Leu-4-nitroanilide Zn2+-Zn2+-enzyme form Sus scrofa
8.4
-
Leu-Gly Mg2+-Zn2+-enzyme form Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Sus scrofa
Mn2+ activates Sus scrofa
Zn2+ zinc-metallopeptidase, two Zn2+ per protomer Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from kidney Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Phe + H2O
-
Sus scrofa Gly + Phe
-
?
Leu-2-naphthylamide + H2O
-
Sus scrofa Leu + 2-naphthylamine
-
?
Leu-4-nitroanilide + H2O
-
Sus scrofa Leu + 4-nitroaniline
-
?
Leu-Gly + H2O
-
Sus scrofa Leu + Gly
-
?
Leu-Phe + H2O
-
Sus scrofa Leu + Phe
-
?
additional information broad substrate specificity Sus scrofa ?
-
?

Subunits

Subunits Comment Organism
hexamer homohexamer Sus scrofa

Synonyms

Synonyms Comment Organism
LAP
-
Sus scrofa
leucine aminopeptidase
-
Sus scrofa
More the enzyme belongs to the peptidase family M17 Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
Leu-4-nitroanilide Zn2+-Zn2+-enzyme form Sus scrofa
0.04
-
Leu-4-nitroanilide Mg2+-Zn2+-enzyme form Sus scrofa
0.5
-
Gly-Phe Zn2+-Zn2+-enzyme form Sus scrofa
270
-
Leu-Phe Zn2+-Zn2+-enzyme form Sus scrofa
1670
-
Leu-Gly Mg2+-Zn2+-enzyme form Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5
-
Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
amastatin
-
Sus scrofa