Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amastatin | natural inhibitor | Sus scrofa | |
Amino acid hydroxamates | - |
Sus scrofa | |
bestatin | natural inhibitor | Sus scrofa | |
L-leucylthiol | - |
Sus scrofa | |
leucinal | - |
Sus scrofa | |
leucine phosphonic acid | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Sus scrofa | |
0.06 | - |
Leu-Phe | Zn2+-Zn2+-enzyme form | Sus scrofa | |
1 | - |
Gly-Phe | Zn2+-Zn2+-enzyme form | Sus scrofa | |
2 | - |
Leu-4-nitroanilide | Mg2+-Zn2+-enzyme form | Sus scrofa | |
4.1 | - |
Leu-4-nitroanilide | Zn2+-Zn2+-enzyme form | Sus scrofa | |
8.4 | - |
Leu-Gly | Mg2+-Zn2+-enzyme form | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Sus scrofa | |
Mn2+ | activates | Sus scrofa | |
Zn2+ | zinc-metallopeptidase, two Zn2+ per protomer | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from kidney | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-Phe + H2O | - |
Sus scrofa | Gly + Phe | - |
? | |
Leu-2-naphthylamide + H2O | - |
Sus scrofa | Leu + 2-naphthylamine | - |
? | |
Leu-4-nitroanilide + H2O | - |
Sus scrofa | Leu + 4-nitroaniline | - |
? | |
Leu-Gly + H2O | - |
Sus scrofa | Leu + Gly | - |
? | |
Leu-Phe + H2O | - |
Sus scrofa | Leu + Phe | - |
? | |
additional information | broad substrate specificity | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | homohexamer | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Sus scrofa |
leucine aminopeptidase | - |
Sus scrofa |
More | the enzyme belongs to the peptidase family M17 | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
Leu-4-nitroanilide | Zn2+-Zn2+-enzyme form | Sus scrofa | |
0.04 | - |
Leu-4-nitroanilide | Mg2+-Zn2+-enzyme form | Sus scrofa | |
0.5 | - |
Gly-Phe | Zn2+-Zn2+-enzyme form | Sus scrofa | |
270 | - |
Leu-Phe | Zn2+-Zn2+-enzyme form | Sus scrofa | |
1670 | - |
Leu-Gly | Mg2+-Zn2+-enzyme form | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | - |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
amastatin | - |
Sus scrofa |